Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.1K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.1K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

826
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
826

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Betulinic acid ameliorates experimental diabetic-induced renal inflammation and fibrosis via inhibiting the activation of NF-κB signaling pathway.

Molecular and cellular endocrinology·2016
Same author

Primary pulmonary T-cell lymphoma mimicking pneumonia: A case report and literature review.

Experimental and therapeutic medicine·2016
Same author

Investigating polymorphisms by bioinformatics is a potential cost-effective method to screen for germline mutations in Chinese familial adenomatous polyposis patients.

Oncology letters·2016
Same author

Discovery and characterization of a novel potent type II native and mutant BCR-ABL inhibitor (CHMFL-074) for Chronic Myeloid Leukemia (CML).

Oncotarget·2016
Same author

Temporal Patterns in Bacterioplankton Community Composition in Three Reservoirs of Similar Trophic Status in Shenzhen, China.

International journal of environmental research and public health·2016
Same author

Expanding CRISPR/Cas9 Genome Editing Capacity in Zebrafish Using SaCas9.

G3 (Bethesda, Md.)·2016

Related Experiment Video

Updated: Nov 12, 2025

Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional x, y, z, and λ Hyperspectral FRET Imaging and Analysis
08:22

Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional x, y, z, and λ Hyperspectral FRET Imaging and Analysis

Published on: October 27, 2020

4.1K

Two-dimensional spectral signal model for chromatic confocal microscopy.

Cheng Chen, Richard Leach, Jian Wang

    Optics Express
    |March 17, 2021
    PubMed
    Summary

    A new two-dimensional spectral signal model for chromatic confocal microscopy (CCM) improves measurement accuracy by considering spectral intensity, wavelength, and displacement. This model enhances performance without needing prior optical design knowledge.

    More Related Videos

    Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
    14:12

    Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

    Published on: December 11, 2021

    5.7K
    Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing
    08:12

    Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

    Published on: March 13, 2013

    13.0K

    Related Experiment Videos

    Last Updated: Nov 12, 2025

    Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional x, y, z, and λ Hyperspectral FRET Imaging and Analysis
    08:22

    Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional x, y, z, and λ Hyperspectral FRET Imaging and Analysis

    Published on: October 27, 2020

    4.1K
    Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
    14:12

    Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

    Published on: December 11, 2021

    5.7K
    Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing
    08:12

    Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

    Published on: March 13, 2013

    13.0K

    Area of Science:

    • Optics and Photonics
    • Microscopy Techniques
    • Metrology

    Background:

    • Chromatic confocal microscopy (CCM) relies on signal processing for accurate measurements.
    • Current spectral models lack displacement-wavelength relationship, limiting their application in optical design.

    Purpose of the Study:

    • To develop a novel two-dimensional spectral signal model for CCM.
    • To investigate the influence of optical setup dimensions and objective lens properties on spectral signals.

    Main Methods:

    • Development of a 2D spectral signal model incorporating intensity, wavelength, and displacement.
    • Analysis of dimensional characteristics, displacement-wavelength relation, and monochromatic aberrations.
    • Experimental validation of the proposed signal model.

    Main Results:

    • The 2D model accurately describes spectral signal characteristics in CCM.
    • It reveals the impact of setup dimensions and objective aberrations.
    • Experimental results confirm the model's effectiveness.

    Conclusions:

    • The developed 2D spectral signal model enhances understanding of CCM signal behavior.
    • It provides a foundation for improving measurement performance without prior optical design knowledge.