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Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
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Axial resolution analysis in compressive digital holographic microscopy.

Ping Su, Da Sun, Jianshe Ma

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    |March 17, 2021
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    Summary

    Digital holographic microscopy with compressive sensing (CDHM) enhances axial resolution by analyzing system elongation effects. This study quantifies resolution limits and demonstrates fiber tomography, improving microscale imaging in biology and fluid dynamics.

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    Area of Science:

    • Optics and Photonics
    • Microscopy
    • Image Reconstruction

    Background:

    • Digital holographic microscopy with compressive sensing (CDHM) is established for tomography.
    • Enhancing axial resolution in CDHM requires further investigation.
    • Elongation effects in digital holography (DH) systems impact resolution.

    Purpose of the Study:

    • To elucidate and enhance axial resolution in CDHM.
    • To analyze the elongation effect in DH systems without paraxial approximation.
    • To investigate the physical significance of the coherence parameter.

    Main Methods:

    • Deduction of accurate formulas for lateral and axial resolutions.
    • Quantification of the elongation effect based on numerical aperture (NA).
    • Tomography of a fiber using a 10x DH microscope with compressive sensing (CS).

    Main Results:

    • The elongation effect is an inherent DH property dependent solely on NA.
    • Theoretical results were experimentally validated through fiber tomography.
    • Distinguished crossed fibers at 36.4 μm and 48.5 μm using the developed guidelines.

    Conclusions:

    • Provides theoretical guidelines for improving axial resolution in CDHM.
    • Demonstrates the potential of CDHM for microscale tomography and observation.
    • Highlights applications in biological and fluid imaging.