Collateral reinnervation of taste buds after chronic sensory denervation: a morphological study

Peripheral transganglionic transport of horseradish peroxidase (HRP) was used to label afferent fibers in the taste buds and lingual epithelium 2-12 weeks after chronic chorda tympani or combined chorda tympani-lingual nerve lesions. From 4-12 weeks after a chronic chorda tympani lesion, taste buds could be found. These were innervated by fibers from the ipsilateral lingual nerve. From 8-12 weeks after a chronic chorda tympani-lingual nerve lesion, nerve fibers from the contralateral lingual nerve could be found in a few taste buds on the denervated side of the tongue. Thus, collateral sprouting took place over the midline in this instance. These findings indicate that intact gustatory axons do not sprout into denervated taste buds, but trigeminal fibers in the lingual nerve do have this ability.