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Related Experiment Video

Updated: Nov 12, 2025

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

Sputum Processing Method for Lateral Flow Immunochromatographic Assays to Detect Coronaviruses.

Aram Kang¹, Minjoo Yeom¹, Hyekwon Kim²

¹College of Pharmacy, Korea University, Sejong 30019, Korea.

|March 17, 2021

Summary

This study presents a new method to improve coronavirus diagnosis in sputum using mucolytic agents. The optimized pretreatment enhances immunochromatographic assay (ICA) sensitivity for detecting viruses like MERS-CoV.

Keywords:

Chromatography, Sensitivity Coronavirus Diagnosis Sputum

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Area of Science:

Virology
Diagnostic Assays
Biochemistry

Background:

Coronaviruses cause zoonotic diseases, necessitating early diagnosis for control.
Immunochromatographic assay (ICA) is a rapid point-of-care diagnostic tool.
Sputum viscosity hinders ICA performance by impeding antigen migration.

Purpose of the Study:

To develop an effective sputum pretreatment method for enhanced coronavirus detection via ICA.
To identify optimal mucolytic agents that reduce sputum viscosity without compromising viral antigens.

Main Methods:

Combined known mucolytic agents to decrease sputum viscosity.
Tested pretreatment efficacy on sputum samples spiked with alpha and beta coronaviruses (PEDV and MERS-CoV).
Evaluated the performance of immunochromatographic assay (ICA) with pretreated samples.

Main Results:

A pretreatment method using tris(2-carboxyethyl)phosphine (TCEP) and Bovine Serum Albumin (BSA) effectively lowered sputum viscosity.
This TCEP and BSA pretreatment significantly improved ICA sensitivity for detecting PEDV and MERS-CoV in sputum.
The method demonstrated suitability for diagnosing lower respiratory tract pathogens.

Conclusions:

Optimized sputum pretreatment using TCEP and BSA enables sensitive ICA diagnosis of coronaviruses.
This approach overcomes viscosity limitations in sputum samples for point-of-care testing.
Provides a valuable tool for rapid diagnosis of lower respiratory tract coronavirus infections.