Extending the post-thaw viability of cryoprecipitate

Area of Science:

• Transfusion Medicine
• Hematology
• Biochemistry

Background:

• Cryoprecipitate has a limited 6-hour post-thaw expiry.
• Assessing extended storage stability is crucial for clinical utility.

Purpose of the Study:

• To evaluate the stability and function of cryoprecipitate components (FVIII, fibrinogen, vWF, FXIII) up to 120 hours post-thaw.
• To assess cryoprecipitate sterility after extended storage at 2-6°C and room temperature (20-24°C).

Main Methods:

• Twenty batches of thawed cryoprecipitate were stored at 2-6°C or room temperature.
• Samples were tested every 24 hours for 120 hours for FVIII, fibrinogen, FXIII, vWF antigen, and vWF activity.
• Sterility testing was performed on units stored for 120 hours.

Main Results:

• Factor VIII (FVIII) and von Willebrand factor ristocetin cofactor (vWF:RCo) levels declined significantly but remained within standards.
• Fibrinogen, von Willebrand factor antigen (vWFag), and Factor XIII (FXIII) levels remained stable and met requirements.
• No bacterial contamination was detected in any units after 120 hours of storage.

Conclusions:

• Extended storage of thawed cryoprecipitate up to 120 hours is viable at both 2-6°C and room temperature.
• Key hemostatic components maintain adequate levels for clinical use.
• Storage at 2-6°C is recommended due to a lower risk of bacterial contamination.