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Related Experiment Video

Updated: Nov 12, 2025

Analysis of Targeted Viral Protein Nanoparticles Delivered to HER2+ Tumors

Analysis of Targeted Viral Protein Nanoparticles Delivered to HER2+ Tumors

Published on: June 18, 2013

Radiolabeled HER2-directed exosomes exhibit improved cell targeting and specificity.

Mahlegha Ghavami¹, Chrysoula Vraka², Virginie Hubert³

¹Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran 14117-13116, Iran.

Nanomedicine (London, England)

|March 17, 2021

Summary

Researchers developed targeted radiolabeled exosomes for detecting HER2-positive cells. Indium-111 oxine ( [¹¹¹In]In-oxine)-labeled exosomes showed efficient labeling and stability, enabling quantitative imaging of HER2 expression.

Keywords:

DARPin HER2 breast cancer imaging radiotracer

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Tracking Bispecific Antibody-Induced T Cell Trafficking Using Luciferase-Transduced Human T Cells

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Area of Science:

Biomedical imaging
Nanotechnology
Cell biology

Background:

Exosomes are nanoscale vesicles with potential diagnostic applications.
Targeted delivery systems are crucial for specific disease detection.
HER2 expression is a key biomarker in certain cancers.

Purpose of the Study:

To establish a method for generating and characterizing radiolabeled exosomes targeted to HER2-positive cells.
To quantitatively detect HER2-positive cells using targeted exosomes.

Main Methods:

Targeted exosomes (T-exos) were radiolabeled using [^99mTc]Tc-HMPAO or [¹¹¹In]In-oxine.
Labeling efficiency and stability were assessed.
HER2-positive and -negative cells were incubated with [¹¹¹In]In-oxine-T-exos.

Main Results:

[¹¹¹In]In-oxine labeling maintained exosome integrity and binding ability.
Achieved 70% labeling efficiency and 78% radiochemical stability over 24 hours.
[¹¹¹In]In-oxine-T-exos demonstrated enhanced uptake in HER2-positive cells compared to untargeted exosomes.

Conclusions:

[¹¹¹In]In-oxine-T-exos represent a promising tool for quantitative imaging of HER2 expression.
This strategy enables targeted detection of HER2-positive cells.
Radiolabeled exosomes offer potential for improved cancer diagnostics.