Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Western Blotting

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

Effects of EDTA on End-Point Detection Methods

Effects of EDTA on End-Point Detection Methods

Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Metabolic profiles of MK-3984 (SARM) in zebrafish using ultra high performance liquid chromatography Q extractive HF hybrid quadrupole orbitrap mass spectrometry (UPLC-QE-HF-MS) for doping control.

The Analyst·2026

Same author

Unraveling the hidden conditions in NiOOH for electrocatalytic oxidation of methanol to formaldehyde with unity Faraday efficiency and selectivity.

Chemical science·2026

Same author

Predicting physical activity types in children aged 3-6 from video data using computer vision.

BMC public health·2026

Same author

The Effects of Different Exercises on Insulin Resistance and Testosterone Changes in Women with Polycystic Ovarian Syndrome: A Network Meta-Analysis Study.

Healthcare (Basel, Switzerland)·2025

Same author

Comprehensive Screening of Multiple Prohibited Substances in Chinese Traditional Patent Medicine by UHPLC-Q-Orbitrap HRMS.

Drug testing and analysis·2025

Same author

Gas chromatography properties and mass spectrometry fragmentation of anabolic androgenic steroids in doping control.

Bioanalysis·2023

Search research articles

Related Experiment Video

Updated: Nov 12, 2025

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Published on: March 13, 2015

Dried blood spots in doping analysis.

Yuling Yuan^1,2, Youxuan Xu², Jianghai Lu²

¹School of Sport Science, Beijing Sport University, No.48 Xinxi Road, Beijing, PR China.

|March 17, 2021

Summary

Dried blood spot (DBS) detection methods offer a less invasive approach to identifying doping agents. This review highlights DBS advancements crucial for routine anti-doping control, especially for major sporting events.

Keywords:

DBS collection anabolic steroids anti-doping derivatization dried blood spots hematocrit mass spectrometry

More Related Videos

Dried Blood and Serum Spots As A Useful Tool for Sample Storage to Evaluate Cancer Biomarkers

Dried Blood and Serum Spots As A Useful Tool for Sample Storage to Evaluate Cancer Biomarkers

Published on: June 11, 2018

Dried Blood Spot Collection of Health Biomarkers to Maximize Participation in Population Studies

Dried Blood Spot Collection of Health Biomarkers to Maximize Participation in Population Studies

Published on: January 28, 2014

Related Experiment Videos

Last Updated: Nov 12, 2025

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Published on: March 13, 2015

Dried Blood and Serum Spots As A Useful Tool for Sample Storage to Evaluate Cancer Biomarkers

Dried Blood and Serum Spots As A Useful Tool for Sample Storage to Evaluate Cancer Biomarkers

Published on: June 11, 2018

Dried Blood Spot Collection of Health Biomarkers to Maximize Participation in Population Studies

Dried Blood Spot Collection of Health Biomarkers to Maximize Participation in Population Studies

Published on: January 28, 2014

Area of Science:

Analytical Chemistry
Sports Science
Biomedical Analysis

Background:

Dried blood spot (DBS) technology has advanced significantly over the last two decades for detecting doping agents.
DBS offers advantages like minimized invasiveness, reduced storage, and lower shipping costs compared to traditional methods.
The World Anti-Doping Agency's adoption of DBS for the 2022 Beijing Winter Olympics underscores its growing importance in anti-doping.

Purpose of the Study:

To review the current state of dried blood spot (DBS) detection methods for doping agents.
To summarize key aspects of DBS application in doping analysis, including collection, storage, pretreatment, instrumentation, and validation.
To provide insights and suggestions for future research in DBS-based doping analysis.

Main Methods:

Literature review focusing on DBS techniques for doping agent detection.
Analysis of DBS application aspects: sample collection, storage conditions, pretreatment, instrumentation, and validation.
Consideration of methods in alignment with the World Anti-Doping Agency's Prohibited List.

Main Results:

DBS methods have been developed and refined over the past twenty years.
The technique offers significant logistical and practical benefits for doping control.
The review covers critical parameters for effective DBS implementation in anti-doping strategies.

Conclusions:

Further development of DBS detection methods for doping agents is essential and urgent.
DBS is a valuable and increasingly adopted technique for routine anti-doping control.
Future research should focus on optimizing DBS protocols for comprehensive doping analysis.