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Published on: September 22, 2019
Complex changes of circadian proteins expression in inflammatory bowel disease
Circadian rhythm disruption may contribute to inflammatory bowel diseases (IBD). This study found decreased core circadian proteins in the intestinal lining of IBD patients, suggesting a link to disease development.
Area of Science:
- Gastroenterology
- Chronobiology
- Immunology
Background:
- The circadian system influences immune activity and gut microbiota.
- These interactions suggest a potential role in inflammatory bowel diseases (IBD).
Purpose of the Study:
- To investigate the expression of core circadian proteins in the intestinal mucosa of IBD patients.
- To explore the association between circadian rhythm deregulation and IBD pathogenesis.
Main Methods:
- Retrospective immunohistochemical analysis of 8 core circadian proteins in intestinal epithelial cells.
- Compared expression in 24 Crohn's disease (CD) and 26 ulcerative colitis (UC) patients versus controls.
- Assessed BMAL1 expression in intramucosal inflammatory cells.
Main Results:
- Reduced expression of BMAL1, PER1, PER3, TIMELESS, and NPAS2 in the mucosal epithelium of IBD patients compared to controls.
- More significant decreases in BMAL1 and PER1 in UC patients, and PER3, TIMELESS, and NPAS2 in CD patients.
- Decreased BMAL1 expression was also observed in intramucosal inflammatory cells of IBD patients.
Conclusions:
- Downregulation of core circadian proteins in the colonic mucosa and inflammatory cells suggests circadian rhythm deregulation contributes to IBD development.
- This study represents an extensive immunohistochemical analysis of circadian protein expression in IBD intestinal mucosa.
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