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Related Concept Videos

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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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Related Experiment Video

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Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution
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Mutant non-coding RNA resource in mouse embryonic stem cells.

Jens Hansen1, Harald von Melchner2, Wolfgang Wurst1,3,4,5

  • 1Institute of Developmental Genetics, Helmholtz Zentrum München GmbH, German Research Center for Environmental Health, Ingolstädter Landstr. 1, D-85764 Neuherberg, Germany hansen@helmholtz-muenchen.de wurst@helmholtz-muenchen.de.

Disease Models & Mechanisms
|March 17, 2021
PubMed
Summary
This summary is machine-generated.

Gene trapping technology has identified thousands of mutations in protein-coding and non-coding RNA genes within a large mouse embryonic stem cell library. This resource enables functional studies of gene disruption and expression.

Keywords:
Gene trapLong non-coding RNAMouseMus musculusMutagenesisMutation

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Area of Science:

  • Genomics
  • Molecular Biology
  • Developmental Biology

Background:

  • Gene trapping is a high-throughput method for creating insertional mutations in mouse embryonic stem (ES) cells.
  • Generic gene trap vectors mutate endogenous genes and report expression, providing DNA tags for gene identification.

Purpose of the Study:

  • To re-investigate a large gene trap library for novel gene disruptions.
  • To identify mutations in non-coding RNA (ncRNA) genes.
  • To analyze gene trap vector integration preferences in relation to long non-coding RNA (lncRNA) biotypes.

Main Methods:

  • Analysis of a library of 566,554 mouse ES cell lines with single gene trap integrations.
  • Identification of disrupted protein-coding and ncRNA genes.
  • Investigation of gene trap vector integration patterns.

Main Results:

  • Identified mutations in 12,078 distinct protein-coding genes.
  • Discovered mutations in 2202 ncRNA genes, including specific long non-coding RNA (lncRNA) genes.
  • Observed preferential integration of certain gene trap vectors into specific lncRNA biotypes.

Conclusions:

  • The study expands the understanding of gene function by identifying numerous mutations in both protein-coding and ncRNA genes.
  • The findings highlight specific gene trap vector biases for lncRNA biotypes.
  • The gene-trapped ES cell lines, including those targeting lncRNAs, are valuable resources for functional research.