Related Experiment Video
Updated: Nov 12, 2025
A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration
Published on: March 10, 2023
A novel quantitative analysis method for idiopathic epiretinal membrane
Davide Allegrini1,2, Giovanni Montesano3,4, Stefania Marconi5
1Department of Biomedical Sciences, Humanitas University, Milan, Italy.
Purpose:
To introduce a novel method to quantitively analyse in three dimensions traction forces in a vast area of the ocular posterior pole.
Methods:
Retrospective analysis of 14 eyes who underwent peeling surgery for idiopathic, symptomatic and progressive epiretinal membrane. The technique measures the shift in position of vascular crossings after surgery from a fixed point, which is the retinal pigmented epithelium. This shift is defined as the relaxation index (RI) and represents a measure of the postoperative movement of the retina due to released traction after surgery.
Results:
Best-corrected visual acuity was significantly better than baseline at all follow ups while the RI had its maximum value at baseline. Moreover, we found a significant correlation between best-corrected visual acuity at 6 months and RI at baseline.
Conclusion:
While all previous published methods focused on bi-dimensional changes observed in a small region, this study introduces a three-dimensional assessment of tractional forces. Future integration of RI into built-in processing software will allow systematic three-dimensional measurement of intraretinal traction.
