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Updated: Nov 12, 2025

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Reversible Ratiometric NADH Sensing Using Semiconducting Polymer Dots.

Haobin Chen1, Jiangbo Yu1, Xiaoxiao Men2

  • 1Department of Chemistry and Bioengineering, University of Washington, Seattle, WA, 98195, USA.

Angewandte Chemie (International Ed. in English)
|March 17, 2021
PubMed
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Researchers developed novel semiconducting polymer dots (Pdots) for sensitive and rapid detection of reduced nicotinamide adenine dinucleotide (NADH). These nanoprobes enable point-of-care NADH sensing, crucial for monitoring cell metabolism and disease states.

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Reduced nicotinamide adenine dinucleotide (NADH) is a vital coenzyme and indicator of cellular metabolic state.
  • Abnormal NADH levels are linked to metabolic diseases and neurodegenerative disorders.
  • There is a need for simple, rapid point-of-care methods for NADH measurement.

Purpose of the Study:

  • To develop and evaluate semiconducting polymer dots (Pdots) as nanoprobes for NADH sensing.
  • To investigate NADH sensing mechanisms based on electron transfer and fluorescence quenching.
  • To enable ratiometric detection of NADH for improved accuracy and sensitivity.

Main Methods:

  • Synthesis of polyfluorene-based and DPA-CNPPV-based semiconducting polymer dots (Pdots).
Keywords:
ratiometric sensorreduced nicotinamide adenine dinucleotide (NADH)semiconducting polymer dots (Pdots)smartphone biosensor

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  • Investigation of fluorescence quenching mechanisms upon interaction with NADH.
  • Development of ratiometric detection strategies using DPA-CNPPV Pdots.
  • In vitro and in vivo performance testing of Pdot nanoprobes.

    • Main Results:

    • Developed Pdots exhibiting NADH-sensitive fluorescence quenching.
    • Achieved ratiometric NADH detection with DPA-CNPPV Pdots.
    • Demonstrated high sensitivity (3.1 μM LOD), selectivity, reversibility, and fast response (<5 s).
    • Showcased smartphone-based NADH imaging for point-of-care applications.

    Conclusions:

    • Semiconducting polymer dots offer a promising platform for sensitive and rapid NADH detection.
    • Ratiometric Pdots provide a robust method for point-of-care NADH sensing.
    • This technology has potential applications in diagnosing and monitoring metabolic and neurodegenerative diseases.