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Staphylococcus aureus--human endothelial cell interactions.

F D Lowy1, J Fant, L L Higgins

  • 1Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10467.

Journal of Ultrastructure and Molecular Structure Research
|February 1, 1988
PubMed
Summary
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Staphylococcus aureus invades human endothelial cells, replicating inside and causing cell death. This study reveals the endothelial cell

Area of Science:

  • Microbiology
  • Cell Biology
  • Pathogen-Host Interactions

Background:

  • Staphylococcus aureus is a dangerous pathogen responsible for severe infections.
  • Endovascular tissue invasion is a key virulence factor for S. aureus.
  • Understanding bacterial entry into endothelial cells is crucial for combating infections.

Purpose of the Study:

  • To investigate the interaction between Staphylococcus aureus and human endothelial cells.
  • To elucidate the mechanisms of S. aureus invasion and intracellular behavior within endothelial cells.
  • To assess the role of endothelial cells in the pathogenesis of endovascular infections.

Main Methods:

  • Ultrastructural analysis of S. aureus interaction with cultured human endothelial cells.
  • Cytochemical staining of lysosomes to examine bacterial degradation.

Related Experiment Videos

  • In vitro infection assays with viable and killed S. aureus to quantify adhesion, phagocytosis, and replication.

    • Main Results:

    • Observed a sequence of staphylococcal invasion: adhesion, endocytosis, and intracellular replication.
    • Endothelial cells underwent disruption and death following S. aureus invasion.
    • Lysosomes fused with intracellular bacteria, but degradation was not observed; viable and killed bacteria adhered similarly, with slower phagocytosis and intracellular replication.

    Conclusions:

    • The endothelial cell plays an active role in the development and dissemination of endovascular S. aureus infections.
    • This in vitro model system is suitable for studying bacterial invasion of the endothelium.
    • S. aureus can survive and replicate within endothelial cells, contributing to disease progression.