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Staphylococcus aureus Responds to Physiologically Relevant Temperature Changes by Altering Its Global Transcript and

Raeven A Bastock1,2,3, Emily C Marino1,4, Richard E Wiemels1

  • 1Department of Biological Sciences, Ohio University, Athens, Ohio, USA.

Msphere
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Summary

Small temperature shifts significantly alter Staphylococcus aureus gene expression, impacting its transition from harmless nasal colonizer to dangerous pathogen. These findings highlight temperature

Keywords:
Staphylococcus aureuscolonizationhost cell invasionposttranscriptional control mechanismsregulationtemperature

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Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Molecular Biology

Background:

  • Staphylococcus aureus is a common nasal colonizer that can cause severe infections.
  • The transition from colonization to infection involves changes in host temperature.
  • Understanding how temperature affects S. aureus is crucial for controlling invasive infections.

Purpose of the Study:

  • To investigate the impact of physiologically relevant temperature changes on S. aureus gene expression.
  • To determine if small temperature alterations influence S. aureus adaptation and pathogenesis.
  • To explore the regulatory mechanisms underlying temperature-dependent gene expression.

Main Methods:

  • Transcriptomics and proteomics analysis of S. aureus at 34°C, 37°C, and 40°C.
  • Phenotypic analyses to assess physiological relevance of observed changes.
  • Investigation of temperature-dependent effects on S. aureus intracellular invasion.

Main Results:

  • Small temperature changes induce large-scale restructuring of the S. aureus transcriptome and proteome.
  • Most gene expression changes inversely correlate with increasing temperature.
  • Evidence suggests post-transcriptional regulation, possibly by sRNA, modulates these changes.
  • S. aureus grown at 34°C exhibited decreased intracellular invasion.

Conclusions:

  • Biologically meaningful temperature alterations significantly influence S. aureus gene expression.
  • Temperature-dependent gene regulation is a key factor in S. aureus's transition from commensal to pathogen.
  • These findings provide insights into S. aureus pathogenesis and potential therapeutic targets.