Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cleavage and Blastulation01:33

Cleavage and Blastulation

48.5K
After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
48.5K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

2.0K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.0K
Zygotic Development And Stem Cell Formation01:10

Zygotic Development And Stem Cell Formation

6.0K
The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
6.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coccidioides meningitis as a cause of low-pressure hydrocephalus: A contemporary case series and management strategy.

Clinical neurology and neurosurgery·2026
Same author

AI-Accelerated Structure Elucidation of Boavistamides A-C, Cyclic Depsipeptides from a Marine Filamentous Cyanobacterium Collected in Cabo Verde.

bioRxiv : the preprint server for biology·2026
Same author

Mediating role of white blood cells in the relationship between the advanced lung cancer inflammation index and serum neurofilament light chain levels: A study based on NHANES 2013-2014 data.

Medicine·2026
Same author

Human primordial germ cell heterogeneity in vitro is associated with distinctive biological states.

Cellular & molecular biology letters·2026
Same author

AI-Accelerated Structure Elucidation of Boavistamides A<b>-</b>C, Cyclic Depsipeptides from a Marine Filamentous Cyanobacterium Collected in Cabo Verde.

Journal of natural products·2026
Same author

DNA methylation reprogramming in marsupial embryos is restricted to the extraembryonic lineage.

Nature communications·2026

Related Experiment Video

Updated: Nov 12, 2025

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
12:09

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Published on: August 10, 2022

6.9K

Modelling human blastocysts by reprogramming fibroblasts into iBlastoids.

Xiaodong Liu1,2,3, Jia Ping Tan1,2,3, Jan Schröder1,2,3

  • 1Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia.

Nature
|March 18, 2021
PubMed
Summary

Researchers reprogrammed fibroblasts into iBlastoids, 3D human blastocyst models. These models mimic early embryonic development and implantation, offering a new tool for studying embryogenesis and infertility treatments.

More Related Videos

Human Blastocyst Biopsy and Vitrification
10:59

Human Blastocyst Biopsy and Vitrification

Published on: July 26, 2019

23.1K
Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells
08:57

Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells

Published on: July 25, 2016

7.8K

Related Experiment Videos

Last Updated: Nov 12, 2025

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
12:09

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Published on: August 10, 2022

6.9K
Human Blastocyst Biopsy and Vitrification
10:59

Human Blastocyst Biopsy and Vitrification

Published on: July 26, 2019

23.1K
Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells
08:57

Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells

Published on: July 25, 2016

7.8K

Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Reproductive Medicine

Background:

  • Current human stem cell models lack the complexity to fully replicate early embryonic development.
  • Understanding early human development is crucial for addressing infertility and developmental disorders.

Purpose of the Study:

  • To create a novel in vitro model of the human blastocyst using reprogrammed cells.
  • To characterize the iBlastoid model for its ability to recapitulate blastocyst architecture and developmental potential.

Main Methods:

  • Fibroblast reprogramming into three-dimensional iBlastoid models.
  • Comprehensive characterization including histology and single-cell transcriptomics.
  • Assessment of iBlastoid capacity to model early implantation events.

Main Results:

  • iBlastoids successfully mimic human blastocyst structure, including inner cell mass and trophectoderm-like cells.
  • Single-cell transcriptomics confirms cell type identity and developmental potential.
  • iBlastoids model key aspects of early implantation and can generate stem cells.

Conclusions:

  • iBlastoids provide a scalable and tractable in vitro model for human blastocyst biology.
  • This model system facilitates research into early human development, gene mutation effects, and embryotoxicity.
  • iBlastoids hold promise for advancing in vitro fertilization therapies and understanding early pregnancy.