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Circ_0005927 Inhibits the Progression of Colorectal Cancer by Regulating miR-942-5p/BATF2 Axis
Chao Yu1, Deguan Li1, Qiang Yan1
1Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022, People's Republic of China.
Cancer Management and Research
|March 18, 2021
Summary
Circular RNA circ_0005927 inhibits colorectal cancer (CRC) progression by sponging microRNA-942-5p (miR-942-5p) to upregulate basic leucine zipper ATF-like transcription factor 2 (BATF2). This circRNA mechanism offers a potential therapeutic strategy for CRC.
Area of Science:
- Molecular Oncology
- RNA Biology
- Cancer Genetics
Background:
- Colorectal cancer (CRC) is a prevalent and aggressive malignancy.
- Circular RNAs (circRNAs) play a role in CRC development.
- The specific function of circ_0005927 in CRC remains to be fully elucidated.
Purpose of the Study:
- To investigate the role of circ_0005927 in colorectal cancer progression.
- To elucidate the underlying molecular mechanism involving microRNA-942-5p (miR-942-5p) and basic leucine zipper ATF-like transcription factor 2 (BATF2).
Main Methods:
- Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot to assess gene and protein expression.
- Cellular assays including colony formation, apoptosis, migration, and invasion assays.
- Dual-luciferase reporter and RNA immunoprecipitation (RIP) assays to confirm molecular interactions.
- In vivo tumor formation assay to evaluate the effect on tumor growth.
Main Results:
- Circ_0005927 and BATF2 were downregulated, while miR-942-5p was upregulated in CRC tissues.
- Overexpression of circ_0005927 suppressed CRC cell proliferation, migration, and invasion, and induced apoptosis.
- Circ_0005927 acted as a sponge for miR-942-5p, which targets BATF2.
- Circ_0005927 overexpression inhibited tumor growth in vivo.
Conclusions:
- Circ_0005927 exerts tumor-suppressive effects in CRC by modulating the circ_0005927/miR-942-5p/BATF2 axis.
- This finding provides a theoretical foundation for circRNA-based therapeutic strategies against CRC.
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