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Neuronotrophic factors released by C6 glioma cells
R Westermann1, M Hardung, D K Meyer
1Department of Anatomy and Cell Biology, University of Marburg, F.R.G.
Journal of Neurochemistry
|June 1, 1988
Summary
Rat glioma cells (C6 cells) secrete multiple neuronotrophic factors (NTFs) essential for neuronal survival. Fetal calf serum is crucial for the synthesis and release of these neurotrophic factors.
Area of Science:
- Neuroscience
- Cell Biology
- Biochemistry
Background:
- Glial cells, including C6 rat glioma cells, release neuronotrophic factors (NTFs) that support neuronal survival.
- Understanding the specific NTFs secreted by C6 cells and the conditions influencing their release is crucial for neurobiology research.
Purpose of the Study:
- To investigate the release of NTFs by C6 cells under various culture conditions.
- To characterize the proteins present in C6 cell conditioned media (C6CMs).
- To determine the role of serum in NTF production and secretion by C6 cells.
Main Methods:
- Analysis of C6CMs using biological, biochemical, and immunological assays.
- Quantification of secreted proteins and assessment of cell death markers.
- Investigation of NTF presence, including laminin and nerve growth factor.
Main Results:
- C6CMs contained secreted proteins, not from cell death, at a rate of 0.5 pg protein/cell/day.
- C6CMs contained laminin and low amounts of nerve growth factor, along with at least six distinct NTFs.
- Fetal calf serum was essential for the synthesis and release of NTFs by C6 cells.
Conclusions:
- C6 cells produce and secrete at least six different NTFs, with nerve growth factor being the only previously established NTF.
- The study identifies C6 cells as a source of multiple neurotrophic factors.
- Serum presence is critical for C6 cell-mediated neurotrophic factor secretion.