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Ultrastructural Study of Cryptococcus neoformans Surface During Budding Events.

Glauber R de S Araújo1,2, Carolina de L Alcantara1, Noêmia Rodrigues1

  • 1Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.

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|March 18, 2021
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Summary

This study reveals how Cryptococcus neoformans remodels its cell surface during budding. These ultrastructural changes in the capsule, cell wall, and membrane may offer new drug targets for cryptococcosis.

Keywords:
Cryptococcus neoformansbuddingcell wallelectron microscopypolysaccharide capsule

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Area of Science:

  • Mycology
  • Cell Biology
  • Infectious Diseases

Background:

  • Cryptococcus neoformans causes life-threatening infections, particularly in immunocompromised individuals.
  • The fungal cell surface comprises the plasma membrane, cell wall, and polysaccharide (PS) capsule.
  • Limited knowledge exists regarding the ultrastructural organization and remodeling of these layers during fungal budding.

Purpose of the Study:

  • To elucidate the ultrastructural organization and morphological remodeling of the Cryptococcus neoformans cell surface during budding.
  • To investigate changes in the PS capsule, cell wall, and plasma membrane during yeast division.

Main Methods:

  • Advanced light and electron microscopy techniques were employed.
  • Detailed ultrastructural analysis of Cryptococcus neoformans during budding events.

Main Results:

  • Observed cell wall deformation creating a specialized polar region that layers and separates.
  • Documented reorganization of the capsular PS, with fibers aligning in the direction of budding for both mother and daughter cells.
  • Identified multilamellar membrane structures formed by daughter cells at the opening between mother and daughter cells.

Conclusions:

  • Provided compelling ultrastructural evidence for significant surface remodeling in Cryptococcus neoformans during budding.
  • Suggests that these remodeled specialized regions could serve as potential drug targets for treating cryptococcosis.