Development of phage-based assay to differentiate ciprofloxacin resistant and sensitive Salmonella Typhimurium

This study was designed to evaluate the possibility of using phage-amplification assay for discriminating between antibiotic-sensitive and antibiotic-resistant Salmonella Typhimurium. The characteristics of Salmonella phage PBST32 were determined by adsorption rate, one-step growth curve, and lytic activity. The ability of phage-based method to detect S. Typhimurium ATCC 19585 (ST^CIP) was determined in single culture and bacterial mixtures of S. Typhimurium ATCC 19585 (ST^WT), Klebsiella pneumoniae, and Staphylococcus aureus. The adsorption rates of PBST32 were 95% and 93% against ST^WT and ST^CIP after 20 min, respectively. The PBST32 showed latent period of 20 min and average burst size of 90 against ST^WT and ST^CIP. The ST^CIP was selectively detected in mixtures of S. aureus, K. pneumoniae, and ST^WT by phage amplification assay. These results provide useful information for designing phage amplification method that can differentially detect antibiotic-resistant pathogens.