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Updated: Nov 12, 2025

Efficient Polyethylene Glycol PEG Mediated Transformation of the Moss Physcomitrella patens
Published on: April 19, 2011
Cellulase and Macerozyme-PEG-mediated Transformation of Moss Protoplasts
Rituraj Batth1, Ilenuta Simina Cuciurean1, Satish Kumar Kodiripaka1
1Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, 2800 Denmark.
Abstract:
This protocol describes the generation of protoplasts from protonemal tissue of the moss Physcomitrium patens (syn. Physcomitrella patens), using Cellulase ONOZUKA R10 and Macerozyme R10, followed by polyethylene glycol (PEG) mediated transformation. The protonemal tissue grown in liquid suspension was harvested and treated with enzyme cocktails mix of 1.5% Cellulase ONOZUKA R10 and 0.5% Macerozyme R10 to generate 1,8 million protoplasts within 3 h.
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