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Related Experiment Videos

Cation effects on cell shape.

M P Sheetz

    Progress in Clinical and Biological Research
    |January 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Cation addition causes rapid, reversible shape changes in human red blood cell ghosts and whole cells. Surface charge removal enhances these cation-induced cell shape alterations.

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    Area of Science:

    • Cell Biology
    • Biophysics
    • Membrane Physiology

    Background:

    • Red blood cell (erythrocyte) membrane integrity and shape are critical for function.
    • Cation interactions with cell membranes can induce significant morphological changes.
    • Understanding these interactions is key to cell physiology and pathology.

    Purpose of the Study:

    • To investigate the effects of various cations on human erythrocyte ghost and whole cell morphology.
    • To elucidate the mechanisms and kinetics of cation-induced shape changes.
    • To explore the role of surface charge in cation-mediated cell shape alterations.

    Main Methods:

    • Incubation of human erythrocyte ghosts and whole cells with different concentrations of monovalent (Na+, K+), divalent (Ca++, Ba++, Sr++, Mg++), and trivalent (La+++) cations.

    Related Experiment Videos

  • Observation of cell shape changes using microscopy.
  • Assessment of reversibility and kinetics of shape changes.
  • Neuraminidase treatment to remove sialic acid residues.
  • Use of ionophore A23187 in conjunction with cations.

    • Main Results:

    • Cation addition (Na+, K+, Ca++, Ba++, Sr++, Mg++, La+++) induced rapid crenation in erythrocyte ghosts.
    • Shape changes were reversible upon cation dilution and could be inhibited by prior incubation at 37°C.
    • Whole red blood cells exhibited cupping (invagination) with divalent and trivalent cations.
    • Neuraminidase treatment facilitated cation-induced shape changes in both ghosts and whole cells.
    • A distinct, slower crenation mechanism was observed in whole cells with A23187 and Ca++/Ba++.

    Conclusions:

    • Cations induce rapid, reversible shape changes in erythrocytes, with mechanisms potentially differing between ghosts and whole cells.
    • Surface charge, particularly sialic acid, plays a significant role in modulating cation-induced erythrocyte shape changes.
    • The study identifies distinct pathways for cation-induced morphological alterations in red blood cells.