Related Experiment Video
Updated: Nov 12, 2025

Author Spotlight: Generation of Patient-Derived Podocytes from Skin Biopsies
Published on: May 26, 2023
The Differentiation of Human Induced Pluripotent Stem Cells into Podocytes In Vitro
Andrea F Wise1, Sheetal Saini1, Sharon D Ricardo2
1Department of Pharmacology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.
Abstract:
Induced pluripotent stem cells (iPSCs) hold enormous potential in the field of regenerative medicine due to their pluripotent properties, where they can give rise to all cell types in the body. Here we describe a detailed 20-day culture and differentiation protocol to generate iPSC-derived podocytes grown as a monolayer. These iPSC-derived podocytes appear arborised by morphology and express podocyte-specific markers. Also described is a detailed immunofluorescence staining protocol to confirm successful differentiation using the podocyte-specific markers, Wilms' tumor protein (WT1) and podocin.
More Related Videos
Related Concept Videos
iPS Cell Differentiation
Induced Pluripotent Stem Cells

