Related Experiment Video

Updated: Nov 12, 2025

In Vivo Assessment of Alveolar Macrophage Efferocytosis Following Ozone Exposure
08:54

In Vivo Assessment of Alveolar Macrophage Efferocytosis Following Ozone Exposure

Published on: October 22, 2019

9.4K

ATP6V0d2 Suppresses Alveoli Macrophage Alternative Polarization and Allergic Asthma via Degradation of PU.1

Na Liu1,2, Yuchen Feng3, Huicheng Liu1

  • 1Department of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology (HUST), Wuhan, China.

Allergy, Asthma & Immunology Research
|March 18, 2021
PubMed
Abstract

Insights

Macrophage ATP6V0d2 acts as a protective factor in asthma by reducing inflammation and mucus. Its deficiency worsens allergic asthma symptoms, highlighting its role in regulating airway inflammation.

Area of Science:

  • Immunology
  • Respiratory Medicine
  • Molecular Biology

Background:

  • Macrophages play a key role in allergic asthma pathogenesis.
  • ATP6V0d2, a vacuolar ATPase subunit, is highly expressed in macrophages, but its function in asthma is unknown.

Purpose of the Study:

  • To investigate the role and molecular mechanisms of macrophage ATP6V0d2 in allergic asthma.
  • To determine if ATP6V0d2 influences airway inflammation, macrophage polarization, and mucus production.

Main Methods:

  • Comparison of disease severity in wild-type versus ATP6V0d2-deficient mice using an ovalbumin-induced asthma model.
  • Analysis of ATP6V0d2, PU.1, and CCL17 expression in asthmatic patients and mouse models.

Main Results:

  • ATP6V0d2 expression was elevated in asthmatic patients and OVA-challenged mice.
  • ATP6V0d2 deficiency exacerbated inflammation, alternative activated macrophage (AAM) polarization, and mucus production in asthma models.
  • ATP6V0d2 promotes lysosomal degradation of PU.1, reducing AAM polarization and CCL17 production.

Conclusions:

  • Macrophage ATP6V0d2 acts as an induced feedback inhibitor of asthma severity.
  • ATP6V0d2 reduces asthma severity by promoting PU.1 degradation, leading to decreased AAM polarization and CCL17 production.
Keywords:
AsthmaPu.1 proteinV-type ATPasealveolar macrophage

More Related Videos

Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages09:09

Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages

Published on: April 20, 2018

24.6K
Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis07:45

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Published on: November 28, 2015

36.1K

Related Concept Videos

Chronic Obstructive Pulmonary Disease-II: Pathophysiology01:20

Chronic Obstructive Pulmonary Disease-II: Pathophysiology

Chronic Obstructive Pulmonary Disease (COPD) pathophysiology is intricate and multifaceted, involving a complex interplay of physiological processes. Understanding these mechanisms is crucial for effectively managing and treating COPD. Here is an in-depth look at the critical elements in the pathophysiology of COPD:
Chronic Inflammation
3.6K
Asthma-II: Pathophysiology and Classification01:26

Asthma-II: Pathophysiology and Classification

Asthma is a prevalent chronic respiratory condition marked by inflammation and hyperresponsiveness of the airways. Its pathophysiology involves complex interactions among inflammatory pathways, immune responses, and neural mechanisms.
Additionally, environmental and genetic factors play crucial roles in determining an individual's susceptibility to asthma and the severity of their condition.
Critical processes in asthma pathophysiology include:
3.5K
Asthma: Pathogenesis and Management01:20

Asthma: Pathogenesis and Management

Asthma is a chronic pulmonary condition involving inflammation of the airways, hyper-reactivity, and reversible obstruction of the airways. This condition can significantly impact a person's quality of life, making breathing difficult and leading to distressing symptoms.
Asthma is classified as allergic and non-allergic. Allergens such as dust mites, pollen, and pet dander trigger allergic asthma, while factors like cold air, intense emotions, or exercise can induce non-allergic asthma.
937
Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs01:25

Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs

Asthma is a chronic respiratory condition for which new therapeutic avenues, including anti-inflammatory drugs like mast cell stabilizers and anti-IgE treatments, continue to be developed.
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...
1.5K
Antiasthma Drugs: Leukotriene Modifiers01:19

Antiasthma Drugs: Leukotriene Modifiers

Leukotriene modifiers, or cysteinyl leukotriene receptor antagonists, are medications used to manage chronic asthma. These agents target specific inflammatory mediators produced during arachidonic acid metabolism, an essential process in generating inflammation in the body.
Leukotriene modifiers work through two distinct mechanisms:
1.5K
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
3.8K