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Colorimetric ratiometry with ion optodes for spatially resolved concentration analysis.

Yoshiki Soda1, Eric Bakker1

  • 1Department of Inorganic and Analytical Chemistry, University of Geneva, Quai Ernest-Ansermet 30, CH-1211, Geneva, Switzerland.

Analytica Chimica Acta
|March 19, 2021
PubMed
Summary

A new ratiometric image analysis method allows for 2D mapping of pH indicator deprotonation in ion optodes. This technique provides spatially resolved ion concentration data, overcoming limitations of traditional spectrophotometry for heterogeneous systems.

Keywords:
ChromoionophoreColorimetryConcentration mappingIon-selective optodeRatiometry

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Area of Science:

  • Analytical Chemistry
  • Chemical Sensing
  • Spectroscopy

Background:

  • Traditional spectrophotometry for measuring pH indicator deprotonation in ion optodes lacks spatial resolution, hindering analysis of heterogeneous systems.
  • Spatially resolved concentration information is crucial for understanding complex chemical environments and processes.

Purpose of the Study:

  • To develop and validate a novel colorimetric method for spatially resolved deprotonation degree measurement in ion optodes.
  • To enable two-dimensional mapping of ion concentrations using image analysis.

Main Methods:

  • A ratiometric image analysis approach was employed using digital image data.
  • The method was tested using a lipophilic pH indicator dye (chromoionophore) in ionophore-based bulk optodes.
  • Potassium ion detection was used as a model system, comparing results with spectrophotometry.

Main Results:

  • The new method successfully maps the deprotonation degree in two dimensions, enabling spatially resolved ion concentration determination.
  • Image analysis-derived deprotonation data quantitatively correlate with spectrophotometric measurements for potassium detection.
  • The method demonstrated independence from camera-specific parameters like gamma correction and spectral sensitivity.

Conclusions:

  • Ratiometric image analysis offers a robust and versatile alternative to spectrophotometry for characterizing ion optodes.
  • This technique provides essential spatially and temporally resolved ion concentration data, particularly for heterogeneous systems.
  • The method is applicable to advanced studies, such as observing ion diffusion dynamics at the pixel level.