Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stabilizing sub-nanoporous zinc metal-organic frameworks in SnTe thermoelectrics for high-temperature power generation.

Chemical science·2026
Same author

Rosindol: A fluorogen for the quantitative measurement of reactive oxygen species in living cells.

bioRxiv : the preprint server for biology·2026
Same author

Coil-Tag Conjugation: A Next Gen Site-Specific ADC Fabrication Technology.

Bioconjugate insights·2026
Same author

Distinct Pathogenic Mechanisms of Two Novel NHS Mutations Identified in Chinese Han Families With Nance-Horan Syndrome.

Human mutation·2026
Same author

Sphingosine-1-phosphate cross-talks to Notch via a S1PR1-Dll4-MPDZ complex to regulate endothelial barrier function.

bioRxiv : the preprint server for biology·2026
Same author

Peptide Tag-nology for Preparation of Site-Specific Antibody-Drug Conjugates.

Bioconjugate chemistry·2026

Related Experiment Video

Updated: Nov 12, 2025

Initial 3D Cell Cluster Control in a Hybrid Gel Cube Device for Repeatable Pattern Formations
05:22

Initial 3D Cell Cluster Control in a Hybrid Gel Cube Device for Repeatable Pattern Formations

Published on: March 21, 2019

5.9K

Controlled Cell Alignment Using Two-Photon Direct Laser Writing-Patterned Hydrogels in 2D and 3D.

Jiaxi Song1, Christos Michas1, Christopher S Chen1

  • 1Department of Biomedical Engineering, Boston University, Boston, MA, 02215, USA.

Macromolecular Bioscience
|March 19, 2021
PubMed
Summary
This summary is machine-generated.

Direct laser writing fabricated microstructures guide cell alignment and adhesion. This technique precisely controls cell positioning on both flat and complex 3D surfaces.

Keywords:
DLWcellular scaffoldshydrogelstissue engineeringtwo-photon polymerization

More Related Videos

Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications
10:45

Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications

Published on: September 29, 2016

13.3K
Fabrication of Micropatterned Hydrogels for Neural Culture Systems using Dynamic Mask Projection Photolithography
16:06

Fabrication of Micropatterned Hydrogels for Neural Culture Systems using Dynamic Mask Projection Photolithography

Published on: February 11, 2011

19.1K

Related Experiment Videos

Last Updated: Nov 12, 2025

Initial 3D Cell Cluster Control in a Hybrid Gel Cube Device for Repeatable Pattern Formations
05:22

Initial 3D Cell Cluster Control in a Hybrid Gel Cube Device for Repeatable Pattern Formations

Published on: March 21, 2019

5.9K
Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications
10:45

Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications

Published on: September 29, 2016

13.3K
Fabrication of Micropatterned Hydrogels for Neural Culture Systems using Dynamic Mask Projection Photolithography
16:06

Fabrication of Micropatterned Hydrogels for Neural Culture Systems using Dynamic Mask Projection Photolithography

Published on: February 11, 2011

19.1K

Area of Science:

  • Biomaterials Engineering
  • Cellular Engineering
  • Microfabrication

Background:

  • Direct laser writing (DLW) via two-photon polymerization is a precise fabrication technique for cellular scaffolds.
  • Controlling microscale cell alignment and selective adhesion on scaffolds is crucial for understanding cell behavior.
  • Existing methods face challenges in patterning cells on complex 3D microstructures.

Purpose of the Study:

  • To develop DLW-fabricated 2D and 3D hydrogel microstructures for controlled cell alignment and selective adhesion.
  • To investigate the use of alternating soft and stiff regions for cell guidance.
  • To demonstrate DLW's capability in patterning cells on intricate surfaces.

Main Methods:

  • Fabrication of 2D and 3D hydrogel microstructures using direct laser writing (DLW) with two-photon polymerization.
  • Incorporation of alternating soft and stiff hydrogel regions.
  • Utilizing cell-adhesive and cell-repellent hydrogels for selective cell patterning.
  • Culturing and observing human mesenchymal stem cells on the fabricated microstructures.

Main Results:

  • Successfully fabricated 2D and 3D hydrogel microstructures with defined soft and stiff regions.
  • Achieved precise control over human mesenchymal stem cell alignment and selective adhesion.
  • Demonstrated DLW patterning of cells on flat, irregular, and curved 3D microstructures.
  • Showcased the ability to direct cell behavior using engineered hydrogel properties.

Conclusions:

  • DLW is a versatile technique for creating advanced cellular scaffolds with patterned mechanical properties.
  • Engineered hydrogel microstructures enable precise control over cell alignment and adhesion.
  • This approach facilitates the study of cell behavior on complex 3D architectures, overcoming previous limitations.