Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Topoisomerase IIIα resolves inter- and intra-molecular intertwines during DNA replication.

bioRxiv : the preprint server for biology·2026
Same author

Dynamic Regulation of Endogenous Transcription Factor Hubs at Single-Molecule Resolution.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Distinct repair outcomes from single and convergent replication fork collapse.

Nature structural & molecular biology·2026
Same author

Dynamic regulation of endogenous transcription factor hubs at single-molecule resolution.

bioRxiv : the preprint server for biology·2026
Same author

Rif1 restrains the rate of replication origin firing in Xenopus laevis.

Communications biology·2023
Same author

Ultrafast and hypersensitive phase imaging of propagating internodal current flows in myelinated axons and electromagnetic pulses in dielectrics.

Nature communications·2022

Related Experiment Video

Updated: Nov 12, 2025

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets
11:19

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Published on: July 7, 2010

14.8K

TopBP1 comes into focus.

William G Dunphy1

  • 1Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.

Molecular Cell
|March 19, 2021
PubMed
Summary
This summary is machine-generated.

Topoisomerase II binding protein 1 (TopBP1) forms nuclear condensates to activate ATR, a key protein in the DNA damage response. This mechanism is crucial for cellular survival during genomic stress.

More Related Videos

Assessing Cellular Target Engagement by SHP2 PTPN11 Phosphatase Inhibitors
08:45

Assessing Cellular Target Engagement by SHP2 PTPN11 Phosphatase Inhibitors

Published on: July 17, 2020

6.4K
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

12.1K

Related Experiment Videos

Last Updated: Nov 12, 2025

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets
11:19

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Published on: July 7, 2010

14.8K
Assessing Cellular Target Engagement by SHP2 PTPN11 Phosphatase Inhibitors
08:45

Assessing Cellular Target Engagement by SHP2 PTPN11 Phosphatase Inhibitors

Published on: July 17, 2020

6.4K
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

12.1K

Area of Science:

  • Cell biology
  • Molecular biology
  • Genetics

Background:

  • Genomic stress triggers cellular responses to maintain genome integrity.
  • ATR (Ataxia Telangiectasia and Rad3-related) is a critical kinase in the DNA damage response pathway.
  • Understanding the regulation of ATR activation is essential for comprehending cellular responses to DNA damage.

Purpose of the Study:

  • To investigate the role of TopBP1 in the activation of ATR.
  • To elucidate the mechanism by which TopBP1 influences ATR activity under genomic stress conditions.

Main Methods:

  • Cellular imaging techniques to visualize nuclear condensates.
  • Biochemical assays to assess ATR kinase activity.
  • Studies using cells experiencing induced genomic stress.

Main Results:

  • TopBP1 forms dynamic, phase-separated nuclear condensates.
  • These TopBP1 condensates are essential for the recruitment and activation of ATR.
  • ATR activation is significantly promoted by TopBP1 condensate formation in stressed cells.

Conclusions:

  • TopBP1-mediated phase separation is a novel mechanism for regulating ATR activation.
  • This process plays a vital role in cellular response to genomic instability.
  • Targeting TopBP1 condensates could offer new therapeutic strategies for diseases involving DNA damage.