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Inside-out chicken enteroids with leukocyte component as a model to study host-pathogen interactions.

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Summary

Researchers developed novel 3D chicken enteroids from embryonic and adult tissues. These avian enteroids offer a unique inside-out model for studying host-pathogen interactions and intestinal biology.

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Area of Science:

  • * Comparative biology
  • * Gastrointestinal research
  • * Avian models

Background:

  • * Mammalian three-dimensional (3D) enteroids are valuable for studying intestinal cell biology and host-pathogen interactions.
  • * Existing models require structural support, limiting certain research applications.

Purpose of the Study:

  • * To develop complex, multilobulated 3D chicken enteroids.
  • * To establish a novel avian intestinal model for research.
  • * To investigate epithelial and leukocyte interactions in chicken intestine models.

Main Methods:

  • * Culturing of 3D chicken enteroids from embryonic villi and adult crypts in suspension.
  • * Histological and transcriptional analyses to characterize enteroid composition.
  • * Infection studies using Salmonella Typhimurium, influenza A virus, and Eimeria tenella.

Main Results:

  • * Developed complex, multilobulated 3D chicken enteroids that grow in suspension.
  • * Enteroids exhibit an inside-out conformation with media-facing apical brush borders.
  • * Enteroids comprise differentiated intestinal epithelial cells, intraepithelial leukocytes, and lamina propria leukocytes.
  • * Demonstrated successful apical surface infection with Salmonella Typhimurium, influenza A virus, and Eimeria tenella.

Conclusions:

  • * Successfully created a comprehensive 3D chicken intestinal model.
  • * The model facilitates the study of epithelial and leukocyte interactions.
  • * This model has significant potential for host-pathogen, food science, and pharmaceutical research.