Related Experiment Video
Updated: Nov 12, 2025
06:28
Leveraging Turbidity and Thromboelastography for Complementary Clot Characterization
Published on: June 4, 2020
7.0K
Added Value of Blood Cells in Thrombin Generation Testing
Jun Wan1,2, Joke Konings1,2, Bas de Laat1,2
1Synapse Research Institute, Maastricht, The Netherlands.
Thrombosis and Haemostasis
|March 20, 2021
Summary
This review explores continuous thrombin generation (TG) tests that incorporate blood cells, offering new insights into coagulation disorders. Further validation is needed for these cell-dependent TG assays in clinical practice.
Area of Science:
- Coagulation Science
- Hematology
- Biomedical Engineering
Background:
- Thrombin generation (TG) is crucial for blood coagulability and has clinical applications.
- Plasma TG tests provide insights into coagulation but exclude the role of blood cells.
- Continuous TG assays offer a more comprehensive view of coagulation dynamics.
Purpose of the Study:
- To review continuous TG tests that measure blood cell involvement in coagulation.
- To highlight the influence of platelets, erythrocytes, and leukocytes on TG.
- To discuss the potential clinical utility of cell-dependent TG assays.
Main Methods:
- Literature review of continuous TG assays, focusing on fluorogenic methods.
- Analysis of studies investigating blood cell contributions to TG.
- Examination of cell-mediated coagulation mechanisms.
Main Results:
- Continuous TG assays, particularly fluorogenic ones, can measure TG in platelet-rich plasma and whole blood.
- Blood cells significantly influence TG: platelets accelerate initiation and velocity, erythrocytes provide phosphatidylserine and trigger contact activation, and leukocytes/cancer cells can express tissue factor.
- Cell-dependent TG assays show promise for distinguishing blood cell-related coagulation disorders.
Conclusions:
- Continuous TG testing in the presence of blood cells offers a more complete picture of coagulation.
- Blood cells play multifaceted roles in accelerating and modulating thrombin generation.
- Clinical validation and standardization of these cell-dependent TG assays are essential for their practical application.
Related Concept Videos
01:20Extrinsic and Intrinsic Pathways of Hemostasis
10.6K
Blood clotting or coagulation involves extrinsic and intrinsic pathways, which ultimately merge into the common pathway, forming a fibrin clot.
The Extrinsic Pathway
The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which...
The Extrinsic Pathway
The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which...
10.6K
01:09Coagulation
8.7K
The coagulation phase is a critical part of the body's process to prevent blood loss following injury to blood vessels. It involves chemical reactions that form a clot to seal the injured area. The clotting process begins shortly after injury, within 15-20 seconds for severe damage and 1-2 minutes for minor injuries.
During the coagulation phase, clotting factors, or procoagulants, play a vital role in initiating and progressing the coagulation cascade. This cascade is a series of reactions...
During the coagulation phase, clotting factors, or procoagulants, play a vital role in initiating and progressing the coagulation cascade. This cascade is a series of reactions...
8.7K
01:30Anticoagulant Drugs: Low-Molecular-Weight Heparins
1.2K
Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
1.2K
01:22Formation of the Platelet Plug
8.0K
The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
8.0K