Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human placenta gangliosides.

T Taki1, K Matsuo, K Yamamoto

  • 1Department of Biochemistry, Shizuoka College of Pharmacy, Japan.

Lipids
|March 1, 1988
PubMed
Summary

Human placenta gangliosides G1-G4 were identified using advanced analytical techniques. Ganglio-series gangliosides were notably absent in placental tissue.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Visual function after foveal translocation with 360-degree retinotomy and simultaneous torsional muscle surgery in patients with myopic neovascular maculopathy.

American journal of ophthalmology·2001
Same author

Features of IgA nephropathy in preschool children.

Clinical nephrology·2001
Same author

Effects of daily oral administration of quercetin chalcone and modified citrus pectin on implanted colon-25 tumor growth in Balb-c mice.

Alternative medicine review : a journal of clinical therapeutic·2001
Same author

Inhibition of experimental choroidal neovascularization by overexpression of tissue inhibitor of metalloproteinases-3 in retinal pigment epithelium cells.

American journal of ophthalmology·2000
Same author

Retinal changes after retinal translocation surgery with scleral imbrication in dog eyes.

Investigative ophthalmology & visual science·2000
Same author

Foveal translocation surgery and myopic subfoveal CNV membrane.

Ophthalmology·2000

Area of Science:

  • Biochemistry
  • Glycobiology
  • Human Placental Biology

Background:

  • Gangliosides are complex glycosphingolipids crucial for cellular functions.
  • Human placenta is a rich source of various bioactive molecules.
  • Understanding placental ganglioside composition is vital for developmental biology.

Purpose of the Study:

  • To isolate and characterize major ganglioside components from human placenta.
  • To elucidate the structural features of identified placental gangliosides.
  • To investigate the presence or absence of ganglio-series gangliosides in the placenta.

Main Methods:

  • Silica beads column chromatography for ganglioside purification.
  • Exoglycosidase treatments for structural analysis.
  • 400 MHz proton magnetic resonance spectroscopy (1H NMR) for structural elucidation.
  • Gas chromatography-mass spectrometry (GC-MS) for compositional analysis.

Main Results:

  • Four major ganglioside components (G1-G4) were isolated and structurally characterized.
  • The structures of G1-G4 were determined, revealing complex sugar residues.
  • Oligosaccharides exceeding 10 sugar residues were detected alongside the major components.
  • Ganglio-series gangliosides were found to be undetectable in the human placenta.

Conclusions:

  • Human placenta contains distinct major gangliosides (G1-G4) with complex structures.
  • The absence of ganglio-series gangliosides suggests specific developmental or functional roles.
  • Further research is needed to understand the biological significance of these placental gangliosides.

Related Experiment Videos