Human placenta gangliosides

Gangliosides extracted from human placenta were composed of four major components (G1-G4), which were purified by silica beads column chromatography. The structures of these gangliosides were analyzed by exoglycosidase treatments, 400 MHz proton magnetic resonance spectroscopy and gas chromatography-mass spectrometry and were concluded to be as follows: (Formula: see text). Gangliosides containing more than 10 sugar residues were observed in addition to the above four major components. But ganglio-series gangliosides were undetectable in the placenta.