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Considerations for brain pH assessment by 31P NMR.

J W Pettegrew1, G Withers, K Panchalingam

  • 1Department of Psychiatry, University of Pittsburgh, PA 15213.

Magnetic Resonance Imaging
|March 1, 1988
PubMed
Summary

This study introduces a new method using 31P NMR spectroscopy to accurately measure brain pH non-invasively. The ATP gamma-alpha chemical shift difference is identified as a reliable indicator for brain pH and intracellular magnesium levels.

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Area of Science:

  • Neuroscience
  • Biochemistry
  • Medical Imaging

Background:

  • Non-invasive in vivo NMR spectroscopy is emerging for brain pH measurement.
  • Current methods using inorganic orthophosphate (Pi) and phosphocreatine (PCr) have limitations in accuracy under various physiological conditions.

Purpose of the Study:

  • To identify a reliable NMR parameter for accurate brain pH measurement across physiological conditions.
  • To evaluate phosphorus-containing metabolites for their suitability as pH indicators, considering linearity, cation independence, and resonance observability.

Main Methods:

  • Systematic 31P NMR study of pH and Mg2+ titration curves for 16 brain phosphorus metabolites.
  • Analysis of chemical shift differences (delta delta) between metabolite resonances.

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Main Results:

  • Phosphoethanolamine (PE)-Pi and PCr-PE delta delta values met linearity and cation independence criteria but lacked consistent observability.
  • The delta delta of ATP gamma-alpha demonstrated linearity, predictable cation effects, and reliable observability across conditions.

Conclusions:

  • The ATP gamma-alpha chemical shift difference is a robust NMR parameter for non-invasive brain pH monitoring.
  • This method also offers potential for estimating intracellular Mg2+ concentration.