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Agarose Gel Electrophoresis for the Separation of DNA Fragments
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Agarose Gel Electrophoresis for the Separation of DNA Fragments

Published on: April 20, 2012

Preparation of agarose gels as reference substances for NMR relaxation time measurement. EEC Concerted Action Program

P Walker1, R A Lerski, R Mathur-De Vré

  • 1Department of Medical Physics, Hammersmith Hospital, University of London, U.K.

Magnetic Resonance Imaging
|March 1, 1988
PubMed

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DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...