Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

478
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
478
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.3K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.3K
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

1.3K
The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
1.3K
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.2K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.2K
Atomic Nuclei: Nuclear Relaxation Processes01:23

Atomic Nuclei: Nuclear Relaxation Processes

891
In the absence of an external magnetic field, nuclear spin states are degenerate and randomly oriented. When a magnetic field is applied, the spins begin to precess and orient themselves along (lower energy) or against (higher energy) the direction of the field. At equilibrium, a slight excess population of spins exists in the lower energy state. Because the direction of the magnetic field is fixed as the z-axis,  the precessing magnetic moments are randomly oriented around the z-axis.
891
Paramagnetism01:30

Paramagnetism

2.8K
Paramagnets are materials with unpaired electrons that possess a finite magnetic moment. In the absence of a magnetic field, these moments are randomly oriented, and thus the net moment is zero. Under an external field, a torque acting on the moments tends to align them along the field's direction. However, the random thermal motion of electrons produces a torque opposite to the external field and tries to disorient the moments. These two competing effects align only a few moments along the...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<sup>1</sup>H R<sub>1ρ</sub> relaxation identifies a hidden intermediate in DNA base-pairing.

Nature communications·2026
Same author

Targeted NMR signal enhancement of RNA by site-directed bis-nitroxide labeling.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Rational Protein Engineering of Branched-Chain α-Keto Acid Decarboxylase for Enhanced 3-Hydroxypropionic Acid Production in <i>Escherichia coli</i>.

ACS synthetic biology·2026
Same author

Computational Modeling and Self-Assembly Synthesis of Borazine-Based Free-Standing Molecular-Thin Films.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Fibrillarin-dependent 2'-O-methylation modulates RPS28 ribosome incorporation and oncogenic translation.

Cancer letters·2025
Same author

BiVO<sub>4</sub>-Cu<sub>2</sub>O/CuO Nanocubes with High Charge Injection and Charge Separation Rates for Enhanced Photoelectrochemical Water Oxidation.

ACS applied energy materials·2025
Same journal

Experimental and computational <sup>11</sup>B NMR comparative study of MOVPE-grown rhombohedral and bulk hexagonal boron nitride.

Solid state nuclear magnetic resonance·2026
Same journal

Determination of <sup>137</sup>Ba nuclear quadrupole interactions in solids: a comparison of high field and zero field approaches.

Solid state nuclear magnetic resonance·2026
Same journal

Probing interlayer bromide in solvent intercalation of layered yttrium hydroxide via <sup>79/81</sup>Br SSNMR spectroscopy.

Solid state nuclear magnetic resonance·2026
Same journal

Single crystal sapphire spacers for in situ angle sensing and rotor stability diagnostics in MAS NMR.

Solid state nuclear magnetic resonance·2026
Same journal

Insights into the local adsorption of CO<sub>2</sub> in UiO-66.

Solid state nuclear magnetic resonance·2026
Same journal

<sup>75</sup>As NQR characterisation of cobaltite (CoAsS).

Solid state nuclear magnetic resonance·2026
See all related articles

Related Experiment Video

Updated: Nov 12, 2025

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
07:24

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

2.0K

Dipolar dephasing for structure determination in a paramagnetic environment.

Rubin Dasgupta1, Karthick B S S Gupta1, Derek Elam1

  • 1Leiden University, Leiden Institute of Chemistry, Gorlaeus Laboratories, Einsteinweg, 55 2333 CC, Leiden, the Netherlands.

Solid State Nuclear Magnetic Resonance
|March 21, 2021
PubMed
Summary
This summary is machine-generated.

REDOR-type sequences effectively measure dipolar coupling in paramagnetic environments. This method provides accurate structural and dynamic insights into paramagnetic complexes, aiding metalloprotein studies.

Keywords:
Cu(II)-(DL-Ala)(2).H(2)ODipolar couplingParamagnetic solid-state NMRREDORShifted-REDOR

More Related Videos

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

15.7K
Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
11:19

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

Published on: July 4, 2016

10.8K

Related Experiment Videos

Last Updated: Nov 12, 2025

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
07:24

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

2.0K
Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

15.7K
Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
11:19

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

Published on: July 4, 2016

10.8K

Area of Science:

  • Solid-state Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Paramagnetic Systems
  • Structural Biology

Background:

  • Determining internuclear distances and molecular dynamics in paramagnetic systems is crucial for understanding metalloprotein active sites.
  • Paramagnetic effects, such as enhanced relaxation and electronic fluctuations, can complicate NMR measurements.
  • REDOR-type sequences offer a potential solution for accurate measurements in these challenging environments.

Purpose of the Study:

  • To demonstrate the efficacy of REDOR-type sequences for measuring dipolar coupling strength in a paramagnetic environment.
  • To utilize paramagnetic effects in Cu(II)-(DL-Ala)2.H2O to estimate C-H dipolar coupling.
  • To assess the utility of this method for obtaining structural and dynamical information from paramagnetic complexes.

Main Methods:

  • Application of REDOR-type sequences to a paramagnetic copper complex (Cu(II)-(DL-Ala)2.H2O).
  • Measurement of dipolar coupling strength for a methyl C-H bond.
  • Analysis of temperature-dependent chemical shifts and 1H-1H spin diffusion spectra.

Main Results:

  • The methyl C-H dipolar coupling was estimated at 8.8 ± 0.6 kHz, scaled by ~0.3 compared to the rigid limit.
  • The derived C-H internuclear distance aligns with crystallographic data.
  • Hyperfine coupling strengths and high-resolution spectra were obtained, demonstrating the method's resolution enhancement.

Conclusions:

  • REDOR-type sequences are effective for determining dipolar coupling strength and providing structural/dynamical information in paramagnetic systems.
  • The observed scaling factor suggests partial averaging of dipolar interactions by methyl group motion.
  • This approach holds promise for high-resolution studies of paramagnetic metalloprotein active sites.