Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mutagenicity and Carcinogenicity01:25

Mutagenicity and Carcinogenicity

1.6K
Mutagenicity and carcinogenicity refer to the ability of drugs to cause genetic defects and induce cancer, respectively. The International Agency for Research on Cancer (IARC) classifies agents into four groups based on their carcinogenic potential. Group 1 agents are known human carcinogens; group 2A agents are probably carcinogenic to humans; group 3 agents lack data to support their role in carcinogenesis; and group 4 includes agents for which data support that they are not likely to be...
1.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prediction of Chronic Kidney Disease Based on Simulated Serum Analysis by Vibrational Spectroscopy.

Biosensors·2026
Same author

The comet assay as a tool for human biomonitoring of exposure to environmental and occupational agents - A summary of systematic reviews and meta-analyses.

Mutation research. Reviews in mutation research·2026
Same author

Pre-Transplant Serum FTIRS Signatures as Predictive Biomarkers of Early Transient Pancreatic Graft Dysfunction in Simultaneous Pancreas-Kidney Transplantation.

Life (Basel, Switzerland)·2026
Same author

Blood biomarker trajectories in ICU-directed prediction models - A scoping review.

The EPMA journal·2026
Same author

Rejection-Focused Precision Medicine in Kidney Transplantation: Biology, Biomarkers, and Artificial Intelligence.

Life (Basel, Switzerland)·2026
Same author

A call for preregistration of in vitro research : Pregistration as a tool to improve reproducibility and transparency.

EMBO reports·2026

Related Experiment Video

Updated: Nov 12, 2025

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
12:15

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

8.9K

A new method to predict genotoxic effects based on serum molecular profile.

Rúben Araújo1, Luís Ramalhete2, Hélder Paz1

  • 1ISEL - Instituto Superior de Engenharia de Lisboa, Instituto Politécnico de Lisboa, Rua Conselheiro Emídio Navarro 1, 1959-007 Lisboa, Portugal.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|March 21, 2021
PubMed
Summary

Fourier Transform Infrared spectroscopy (FTIR) analysis of serum can rapidly detect genotoxic effects from occupational exposure to cytostatic drugs. This method offers a simple, high-throughput screening tool for genotoxic substance exposure in biomonitoring.

Keywords:
BiomonitoringCBMNFTIR spectroscopyGenotoxicitySerum

More Related Videos

The Lambda Select cII Mutation Detection System
07:08

The Lambda Select cII Mutation Detection System

Published on: April 26, 2018

8.1K
Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans
08:14

Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans

Published on: April 28, 2023

583

Related Experiment Videos

Last Updated: Nov 12, 2025

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
12:15

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

8.9K
The Lambda Select cII Mutation Detection System
07:08

The Lambda Select cII Mutation Detection System

Published on: April 26, 2018

8.1K
Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans
08:14

Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans

Published on: April 28, 2023

583

Area of Science:

  • Biomonitoring
  • Occupational Health
  • Spectroscopy

Background:

  • Conventional genotoxicity assessment methods are often laborious, time-consuming, and expensive.
  • Developing rapid and cost-effective methods for assessing genotoxic effects is critical for human biomonitoring.
  • Occupational exposure to cytostatic drugs poses a risk to healthcare professionals.

Purpose of the Study:

  • To evaluate Fourier Transform Infrared (FTIR) spectroscopy of serum for assessing genotoxic effects in hospital professionals occupationally exposed to cytostatic drugs.
  • To compare FTIR serum analysis with the established lymphocyte cytokinesis-block micronucleus assay for genotoxicity.
  • To determine the potential of FTIR spectroscopy as a screening tool for genotoxic exposure.

Main Methods:

  • Peripheral blood samples were collected from hospital professionals exposed (n=22) and not exposed (n=36) to cytostatic drugs.
  • Genotoxicity was assessed using the lymphocyte cytokinesis-block micronucleus assay.
  • Serum samples were analyzed using Fourier Transform Infrared (FTIR) spectroscopy.
  • Hierarchical cluster analysis and support vector machine (SVM) models were applied to serum spectra data.

Main Results:

  • Exposed workers showed a significantly higher number of micronuclei in lymphocytes compared to the non-exposed group (p < 0.05).
  • FTIR spectra of serum from exposed workers exhibited distinct peaks compared to the non-exposed group (p < 0.01).
  • Hierarchical cluster analysis differentiated exposed from non-exposed groups with 61% sensitivity and 88% specificity.
  • An SVM model accurately predicted exposure status with high performance metrics (accuracy: 0.91, precision: 0.89, sensitivity: 0.86, F1 score: 0.87, AUC: 0.96).

Conclusions:

  • FTIR spectroscopic analysis of serum provides a rapid and simple method to predict genotoxic effects from cytostatic drug exposure.
  • This technique demonstrates potential for high-throughput screening of occupational exposure to genotoxic substances.
  • FTIR serum analysis offers a promising alternative or complementary tool to conventional genotoxicity assays in biomonitoring programs.