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Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
Published on: March 28, 2013
Rosiglitazone-induced changes in the oxidative stress metabolism and fatty acid composition in relation with trace
Duygu Aydemir1, Ehsan Sarayloo1, Ulusu Nuriye Nuray1
1Koç University, School of Medicine, Rumelifeneri Yolu, Sariyer, Istanbul, Turkey.
Background:
Metabolic syndrome, obesity and type 2 diabetes are metabolic disorders characterized by the insulin resistance and the impairment in the insulin secretion. Since impairment in the oxidative stress and adipocyte metabolism contribute to the formation of obesity and diabetes, targeting adipose tissue can be considered as an effective approach to fight against them. Rosiglitazone is used for treatment for patients with type 2 diabetes via inducing lipogenesis and transdifferentiation of white adipose tissue into brown adipose tissue. Since the development of such therapeutics is required to control the formation and function of brown fat cells, we aimed to reveal possible molecular mechanisms behind rosiglitazone induced biochemical changes in the adipose tissue.
Methods:
Cells were expanded in the adipocyte culture medium supplemented with 5 µg/mL insulin following 2 days' induction. After those cells were treated with rosiglitazone 0, 0.13 mol/L and 10 µmol/L rosiglitazone for 48 hours and at 8th day, cells were collected and stored at -80 °C. Then the cells were used to evaluate antioxidant enzyme activities, mineral and trace element levels and fatty acid composition.
Results:
Glucose-6-phosphate dehydrogenase and glutathione reductase significantly reduced in rosiglitazone-treated groups compared to the control. Na, Mg, K, Ca, Cr, Fe, Ni, Cu, Zn, Rb, Sr, Cs, Ba and Pb were determined in the cell lysates via ICP-MS. Also, relative FAME content decreased in the rosiglitazone-treated groups compared to the control.
Conclusions:
Rosiglitazone treatment at low doses showed promising results which may promote brown adipose tissue formation.
Insights
Low-dose rosiglitazone treatment reduced antioxidant enzymes and fatty acid content in adipose cells. This suggests potential for promoting brown adipose tissue formation, offering new therapeutic avenues for metabolic disorders.
Area of Science:
- Biochemistry
- Metabolic disorders
- Adipose tissue biology
Background:
- Metabolic syndrome, obesity, and type 2 diabetes involve insulin resistance and impaired insulin secretion.
- Oxidative stress and adipocyte metabolism dysfunction contribute to obesity and diabetes.
- Targeting adipose tissue, particularly brown fat cell formation, is a therapeutic strategy.
Purpose of the Study:
- To investigate the molecular mechanisms of rosiglitazone-induced biochemical changes in adipose tissue.
- To assess the impact of rosiglitazone on antioxidant enzymes, mineral/trace element levels, and fatty acid composition.
Main Methods:
- Adipocyte cell cultures were induced and treated with varying concentrations of rosiglitazone (0, 0.13 µmol/L, 10 µmol/L).
- Cells were collected after 48 hours of treatment for biochemical analysis.
- Evaluated antioxidant enzyme activities, mineral and trace element levels (ICP-MS), and fatty acid methyl ester (FAME) content.
Main Results:
- Rosiglitazone treatment significantly reduced glucose-6-phosphate dehydrogenase and glutathione reductase activities.
- Multiple minerals and trace elements (Na, Mg, K, Ca, Cr, Fe, Ni, Cu, Zn, Rb, Sr, Cs, Ba, Pb) were quantified.
- Relative fatty acid methyl ester (FAME) content decreased in rosiglitazone-treated groups.
Conclusions:
- Low-dose rosiglitazone treatment demonstrates potential in promoting brown adipose tissue (BAT) formation.
- Observed biochemical changes may underlie rosiglitazone's therapeutic effects on metabolic disorders.
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