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Long non-coding RNA PTCSC3 inhibits human oral cancer cell proliferation by inducing apoptosis and autophagy

Hongmei Zhang1, Jiang Wang2, Wenxing Xun1

  • 1Department of Stomatology, the Second Affiliated Hospital of Air Force Medical University, Xi'an, China.

Archives of Medical Science : AMS
|March 22, 2021
PubMed
Abstract

Insights

Long non-coding RNA PTCSC3 acts as a tumor suppressor in oral cancer. It inhibits cancer cell proliferation by inducing apoptosis and autophagy, offering potential therapeutic targets.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer.
  • LncRNA PTCSC3 exhibits tumor-suppressive functions in thyroid cancer and glioblastoma.
  • The function of lncRNA PTCSC3 in human oral cancer remains to be fully elucidated.

Purpose of the Study:

  • To investigate the role of lncRNA PTCSC3 in human oral cancer.
  • To determine the impact of PTCSC3 on oral cancer cell proliferation, apoptosis, and invasion.

Main Methods:

  • MTT assay for cell viability.
  • DAPI and Annexin V/PI assays for apoptosis.
  • Electron microscopy for ultrastructural analysis.
  • Transwell assay for cell invasion.
  • Western blotting for protein expression (LC3B-I, Beclin 1, Bax, Bcl-2).

Main Results:

  • PTCSC3 expression was significantly suppressed in oral cancer tissues and cell lines.
  • PTCSC3 overexpression reduced oral cancer cell proliferation by inducing apoptosis (increased Bax, decreased Bcl-2) and autophagy (increased LC3B-I, Beclin 1).
  • PTCSC3 overexpression significantly decreased oral cancer cell invasion by 62% (SCC-1) and 69% (SCC-9).

Conclusions:

  • LncRNA PTCSC3 functions as a tumor suppressor in human oral cancer.
  • PTCSC3 suppresses oral cancer proliferation through the induction of apoptosis and autophagy.
  • PTCSC3 holds potential as a therapeutic target for oral cancer.
Keywords:
PTCSC3apoptosisautophagylncRNAoral cancer

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