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Effects of persistent lymphocytic choriomeningitis virus infection on cholinergic neurons in the mouse
B R Zisman1, V Braunstein, Y Kress
1Department of Microbiology, Albert Einstein College of Medicine, Bronx, NY 10461.
Abstract:
We have examined the effects of persistent infection with the Armstrong E350 strain of lymphocytic choriomeningitis virus (LCMV) on choline acetyltransferase activity in several regions of Balb/c mouse brain. Despite the presence of high titres of virus in brain for as long as 6 months, and a widespread distribution of virus antigen, no decreases in choline acetyltransferase activity could be demonstrated. The enzyme activity was increased in some regions of brain, showing an effect of the persistent virus infection on a differentiated cell function. Although these data do not suggest a role for LCMV in human neurologic disease, similar studies may allow useful animal models to be conveniently and reproducibly generated.
Insights
Persistent lymphocytic choriomeningitis virus (LCMV) infection in mice brains did not decrease choline acetyltransferase activity. Enzyme activity increased in some brain regions, indicating virus effects on cell function.
Area of Science:
- Neurovirology
- Enzyme kinetics
- Mouse models
Background:
- Persistent viral infections can impact host cell function.
- Lymphocytic choriomeningitis virus (LCMV) is a known neurotropic virus.
- Choline acetyltransferase (ChAT) is a key enzyme in acetylcholine synthesis.
Purpose of the Study:
- To investigate the impact of persistent LCMV infection on ChAT activity in the mouse brain.
- To determine if chronic viral presence affects cholinergic neurotransmission.
Main Methods:
- Balb/c mice were persistently infected with the Armstrong E350 strain of LCMV.
- Choline acetyltransferase activity was measured in various brain regions.
- Virus antigen distribution and titres were assessed.
Main Results:
- High titres and widespread antigen of LCMV were detected in the brain for up to 6 months.
- No reduction in choline acetyltransferase activity was observed despite persistent infection.
- Choline acetyltransferase activity showed an increase in specific brain regions.
Conclusions:
- Persistent LCMV infection does not impair choline acetyltransferase activity in the mouse brain.
- The findings suggest LCMV can alter differentiated cell function, leading to increased enzyme activity in certain areas.
- While not indicating a role in human neurological disease, LCMV models offer reproducible research platforms.