Related Experiment Video

Updated: Nov 12, 2025

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells
11:15

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Published on: May 6, 2018

10.4K

C3AR1 mRNA as a Potential Therapeutic Target Associates With Clinical Outcomes and Tumor Microenvironment in

Tiannan Zou1,2,3, Weibing Liu1, Zeyu Wang1,3

  • 1Department of Orthopedic Surgery, The First People's Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, China.

Frontiers in Medicine
|March 22, 2021
PubMed

Insights

Osteosarcoma treatment may be improved by targeting C3AR1 messenger RNA (mRNA). Low C3AR1 mRNA levels correlate with poor prognosis and increased metastasis, suggesting C3AR1 as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Immunology

Background:

  • Targeting cancer-specific messenger RNAs (mRNAs) presents novel therapeutic strategies for osteosarcoma.
  • Understanding the role of specific mRNAs in osteosarcoma progression and the tumor microenvironment is crucial.

Purpose of the Study:

  • To identify osteosarcoma-specific mRNAs and investigate their biological functions.
  • To evaluate the prognostic value of C3AR1 mRNA in osteosarcoma.
  • To explore the association between C3AR1 mRNA and the tumor immune microenvironment.

Main Methods:

  • Utilized mRNA sequencing data from the TARGET database to analyze gene expression.
  • Employed ESTIMATE and CIBERSORT algorithms to assess stromal/immune scores and immune cell infiltration.
  • Validated C3AR1 mRNA expression and function through RT-qPCR, western blot, and functional assays.

Main Results:

  • Low stromal and immune scores were associated with unfavorable osteosarcoma patient outcomes.
  • C3AR1 mRNA was found to be downregulated in osteosarcoma tissues and correlated with better prognosis.
  • Overexpression of C3AR1 suppressed osteosarcoma cell proliferation, migration, invasion, and induced apoptosis.

Conclusions:

  • C3AR1 mRNA is a potential prognostic biomarker for osteosarcoma.
  • C3AR1 mRNA plays a significant role in regulating the tumor microenvironment and cancer cell behavior.
  • C3AR1 mRNA represents a promising therapeutic target for osteosarcoma treatment.
Keywords:
C3AR1mRNAosteosarcomaprognosistherapeutic targettumor immune microenvironment

More Related Videos

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

7.4K
A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate07:31

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate

Published on: May 3, 2021

4.0K

Related Concept Videos

Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
8.1K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.4K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
22.9K
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
5.7K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.0K
The Tumor Microenvironment02:17

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
7.2K