Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Scavenging multiple reactive carbonyl species simultaneously by mangiferin and its aglycone norathyriol in vitro and in vivo.

Food research international (Ottawa, Ont.)·2026
Same author

Continuous monitoring of glutamate using electroactive templated polymers as synthetic molecular receptors.

The Analyst·2026
Same author

Clinical effectiveness of intraoperative continuous pump infusion of remimazolam on postoperative delirium in elderly patients with hip fractures.

Experimental and therapeutic medicine·2025
Same author

Functional Design and Biophysical Characterization of Analyte-Responsive Polymers.

Biomacromolecules·2025
Same author

Thermodynamic and Kinetic Characterization of Colloidal Polymers of <i>N</i>-Isopropylacrylamide and Alkyl Acrylic Acids for Optical pH Sensing.

Molecules (Basel, Switzerland)·2025
Same author

Ergothioneine, a New Acrolein Scavenger at Elevated Temperature.

Journal of agricultural and food chemistry·2025

Related Experiment Video

Updated: Nov 12, 2025

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

12.3K

Stimuli-Responsive Templated Polymer as a Target Receptor for a Conformation-based Electrochemical Sensing Platform.

Habib M N Ahmad1, Gaurab Dutta1, John Csoros2

  • 1Department of Electrical and Computer Engineering, University of New Hampshire, Durham, NH 03824, United States.

ACS Applied Polymer Materials
|March 22, 2021
PubMed
Summary

We developed a novel single-chain polymer receptor for selective target molecule detection. This stimuli-responsive polymer changes conformation upon binding, enabling label-free electrochemical sensing with faster response times.

Keywords:
Conformation ChangeElectrochemical SensingMolecular ImprintingNitrophenolPoly(N-isopropylacrylamide)RAFTStimuli-ResponsiveTemplated Polymer

More Related Videos

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes
09:28

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

Published on: January 10, 2017

8.3K
Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis
08:46

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

8.0K

Related Experiment Videos

Last Updated: Nov 12, 2025

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

12.3K
Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes
09:28

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

Published on: January 10, 2017

8.3K
Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis
08:46

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

8.0K

Area of Science:

  • Materials Science
  • Polymer Chemistry
  • Analytical Chemistry

Background:

  • Highly crosslinked polymers limit accessibility and require separate signal transduction.
  • Developing efficient, label-free synthetic receptors is crucial for advanced sensing platforms.

Purpose of the Study:

  • To create a single-chain, stimuli-responsive polymer as a target recognition element.
  • To develop a label-free electrochemical sensing platform using this polymer.

Main Methods:

  • Synthesized a poly-N-isopropylacrylamide (PNIPAM)-based copolymer templated with 4-nitrophenol (4-NP).
  • Anchored the copolymer to an electrode surface for electrochemical sensing.
  • Analyzed electrode surface chemistry and performed electrochemical impedance spectroscopy.

Main Results:

  • The single-chain polymer showed preferential binding to the templated target (4-NP).
  • Target binding induced a conformational change in the polymer, altering electrode charge transfer kinetics.
  • Polymer concentration, interchain interactions, and Hofmeister effect influenced grafting and morphology.

Conclusions:

  • Single-chain, stimuli-responsive polymers offer an efficient alternative to crosslinked receptors.
  • This approach enables label-free electrochemical sensing with target-induced conformational changes.
  • The developed platform demonstrates selective detection of 4-nitrophenol.