Co-fractionation/mass spectrometry to identify protein complexes
Claire D McWhite1, Ophelia Papoulas1, Kevin Drew1
1Department of Molecular Biosciences and the Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX 78712, USA.
Abstract:
Co-fractionation/mass spectrometry (CF/MS) is a flexible and powerful method to detect physical associations of proteins. CF/MS can be applied to any tissue or organism without the need for protein-specific antibodies or epitope tags. Here, we outline two alternate protocols for MS preparation of samples (containing low or high salt) and a computational pipeline (cfmsflow) that together allow the successful application of this approach. These protocols are based on CF/MS of over 16 diverse organisms including plants and animals. For complete details on the use and execution of this protocol, please refer to McWhite et al. (2020).
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