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Updated: Nov 12, 2025
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Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
Published on: March 31, 2017
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A practical, low-cost, short-term storage method for genomic DNA.
1Department of Agricultural Biotechnology, Faculty of Agriculture, Akdeniz University, 07070 Antalya, Turkey.
Biotechniques
|March 22, 2021
Summary
Cellulose paper towel and blotting paper effectively preserve DNA for 130 days at room temperature. These low-cost materials offer reliable DNA preservation without degradation, serving as viable alternatives to commercial products.
Area of Science:
- Molecular Biology
- Forensic Science
- Biotechnology
Background:
- DNA preservation is crucial for various applications, including diagnostics and forensics.
- Commercial DNA preservation products can be expensive.
- Low-cost alternatives for DNA storage are needed.
Purpose of the Study:
- To evaluate the DNA preservation capabilities of cellulose paper towel and blotting paper.
- To compare these materials against commercial DNA preservation products.
- To determine the effectiveness of these materials over 130 days at room temperature.
Main Methods:
- Chicken blood was used as the DNA source.
- Blood spots were applied to paper towel, blotting paper, FTA® cards, and DNA/RNA Shield™.
- Samples were stored at room temperature for 130 days.
- DNA extraction was performed using Tris-EDTA and NaOH protocols.
- PCR activity and DNA yield were assessed.
Main Results:
- Paper towel and blotting paper demonstrated significant DNA binding and preservation capacity.
- Reliable DNA extraction and PCR activity were observed from samples stored on paper towel.
- No DNA degradation was detected after 130 days of storage at room temperature.
Conclusions:
- Cellulose-based blotting paper and paper towel are effective and low-cost materials for DNA preservation.
- These materials can maintain DNA integrity for at least 130 days at room temperature.
- Paper towel and blotting paper are suitable alternatives to commercial DNA preservation solutions.
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