Marinobacter orientalis sp. nov., a thiosulfate-oxidizing bacterium isolated from a marine solar saltern

A facultatively anaerobic bacterium, strain W62^T, was isolated from the marine solar saltern in Weihai, China. Cells of the novel strain were Gram-stain negative, non-flagellated, non-gliding, rod-shaped and around 0.3-0.5 × 2.5-3.9 µm in size. Optimum growth occurred at 33-37 °C, with 3-5% (w/v) NaCl and at pH 7.0-7.5. On the basis of phylogenetic analysis of the 16S rRNA gene sequence, strain W62^T had close relationship with Marinobacter vulgaris F01^T (98.6%), Marinobacter confluentis KCTC 42705^T (98.4%) and Marinobacter halotolerans NBRC 110910^T (97.7%). Genome sequencing revealed a genome size of 4,050,555 bp, a G+C content of 57.3% and a complete sox system related to thiosulfate oxidization. Strain W62^T had ubiquinone-9 as the sole respiratory quinone and possessed Summed Features 3 (C_16:1 ω7c/C_16:1 ω6c), C_16:0 and C_18:1 ω9c as the major fatty acids. The major polar lipids of strain W62^T were identified as aminophospholipid, phosphatidylglycerol and phosphatidylethanolamine. According to the results of the phenotypic, chemotaxonomic characterization, phylogenetic properties and genome analysis, strain W62^T should represent a novel specie of the genus Marinobacter, for which the name Marinobacter orientalis sp. nov. is proposed. The type strain is W62^T (= MCCC 1H00317^T = KCTC 62593^T).