Comparison of commercial diluents for holding turkey semen 24 hours at 5 C

The ability was examined of three commercial turkey semen diluents, Beltsville Poultry Semen Extender II (BPSE), Instruments for Veterinary Medicine (IMV), and Minnesota Turkey Growers Association (MTGA) and two diluents containing antibiotics, BPSE + tobramycin (T) and MTGA + gentamicin (G), to maintain the fertilizing capacity of turkey semen held for 24 h at 5 C. Hens were inseminated weekly with 200 million viable spermatozoa for 15 wk. Fertility of unstored, diluted (1:1) semen in MTGA + G (90%) was significantly lower than semen diluted in BPSE (95%) or IMV (95%). Fertilizing capacity of spermatozoa diluted in BPSE + T (94%) and MTGA (91%) was not different from that of semen diluted in the other diluents. Fertility of semen stored for 24 h was highest stored in BPSE (77%) followed by values for storage in BPSE + T (64%), MTGA + G (60%), MTGA (41%), and IMV (38%). Fertility was affected by a significant interaction between semen diluent and storage time. When compared with effects of storage in both Beltsville extenders, spermatozoa stored in the other diluents were less motile. Spermatozoa stored in all extenders except BPSE had a high rate of lysis.