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Updated: Nov 11, 2025

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A highly selective switch-on fluorescence sensor targeting telomeric dimeric G-quadruplex.

Jingfang Zhao1, Qianqian Zhai1

  • 1Department of Chemistry, College of Science, Huazhong Agricultural University, Wuhan 430070, China.

Bioorganic & Medicinal Chemistry Letters
|March 23, 2021
PubMed
Summary

Researchers developed a novel fluorescence probe, HPTA-1, for specific detection of telomeric dimeric G-quadruplexes (TTA45). This probe offers high sensitivity and selectivity, crucial for understanding G-quadruplex related biological processes.

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Area of Science:

  • Biophysical Chemistry
  • Molecular Biology
  • Chemical Biology

Background:

  • Telomeric G-quadruplexes are crucial DNA structures with implications in aging and cancer.
  • Developing selective probes for specific G-quadruplex structures, like telomeric dimeric G-quadruplexes (TTA45), remains a significant challenge.
  • Existing probes often lack the required specificity and sensitivity for accurate G-quadruplex recognition.

Purpose of the Study:

  • To develop a highly specific and sensitive fluorescence probe for the telomeric dimeric G-quadruplex TTA45.
  • To investigate the binding mechanism and sensing capabilities of the probe with TTA45.
  • To establish a reliable method for discriminating TTA45 from other DNA structures using fluorescence.

Main Methods:

  • Utilized a commercially available triazine derivative, HPTA-1, as a fluorescence probe.
Keywords:
A triazine derivative HPTA-1Fluorescence probeRecognition specificitySimple structureTelomeric dimeric G-quadruplexes

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  • Employed fluorescence spectroscopy to monitor the light-up response upon binding to TTA45.
  • Conducted studies to assess selectivity against various DNA structures and evaluated probe-DNA interactions.
  • Investigated the binding mechanism through analysis of electrostatic and π-π stacking interactions.

    • Main Results:

    • HPTA-1 demonstrated a specific fluorescence light-up response for the telomeric dimeric G-quadruplex TTA45.
    • The probe exhibited high selectivity, distinguishing TTA45 from other DNA structures with a significant increase in emission intensity.
    • HPTA-1 maintained the conformation and stability of TTA45 upon binding.
    • Binding studies revealed that electrostatic and π-π stacking interactions between HPTA-1 and TTA45 mediate the specific recognition.

    Conclusions:

    • HPTA-1 is an effective and specific small-molecule fluorescence probe for detecting telomeric dimeric G-quadruplex TTA45.
    • The probe's simple structure and low molecular weight offer advantages over existing multimeric G-quadruplex ligands.
    • This work provides a valuable tool for studying telomeric G-quadruplexes and their biological roles.