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Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

747
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
747
Electrophoresis: Overview01:20

Electrophoresis: Overview

3.0K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
3.0K
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

595
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
595
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

6.8K
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
6.8K
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

2.5K
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
2.5K
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

1.2K
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
1.2K

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Related Experiment Video

Updated: Nov 11, 2025

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
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Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

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Recent (2018-2020) development in capillary electrophoresis.

Ziting Gao1, Wenwan Zhong2

  • 1Department of Chemistry, University of California-Riverside, 900 University Ave., Riverside, CA, 92521, USA.

Analytical and Bioanalytical Chemistry
|March 23, 2021
PubMed
Summary

This review highlights recent advances in capillary electrophoresis (CE), focusing on new methods and instruments for sample preconcentration, separation media, and detection. These developments enhance CE

Keywords:
Capillary coatingsCapillary electrophoresisDetectorsPreconcentrationSeparation media and additives

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Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
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Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
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Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

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Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
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Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Biochemistry

Background:

  • Capillary electrophoresis (CE) offers unique advantages over other separation techniques.
  • CE methodology and instrumentation are continually evolving research areas.
  • Recent advancements aim to expand CE's applicability to novel challenges.

Purpose of the Study:

  • To review representative works in CE published between 2018-2020.
  • To highlight key developments in CE methodology and instrumentation.
  • To showcase applications of CE in solving new analytical problems.

Main Methods:

  • Focus on sample preconcentration techniques in CE.
  • Review of novel separation media and capillary coating developments.
  • Exploration of advanced detector construction and multidimensional separation strategies in CE.

Main Results:

  • Identification of significant trends in CE research from 2018-2020.
  • Demonstration of improved sensitivity through preconcentration methods.
  • Showcasing of enhanced separation efficiency via new media and multidimensional approaches.

Conclusions:

  • CE remains a dynamic field with ongoing innovation.
  • Recent advancements significantly improve CE's performance and scope.
  • The reviewed works underscore CE's potential for addressing complex analytical tasks.