Related Experiment Video
Updated: Nov 11, 2025

Author Spotlight: Innovating Thiol Quantification and Biomarker Detection for Oxidative Stress Research
Published on: June 28, 2024
Hexagonal boron nitride nanoparticles trigger oxidative stress by modulating thiol/disulfide homeostasis
F Kar1,2, I Söğüt3, C Hacıoğlu4
1Department of Medical Services and Techniques, Vocational School of Health Services, Kütahya Health Science University, Kütahya, Turkey.
Hexagonal boron nitride nanoparticles (hBN NPs) can cause oxidative stress in rats at higher doses, impacting serum thiol/disulfide balance. This study reveals hBN NP interactions and effects in vivo for the first time.
Area of Science:
- Biomedical Engineering
- Nanomaterials Science
- Toxicology
Background:
- Hexagonal boron nitride nanoparticles (hBN NPs) show promise in medicine due to unique properties.
- Optimal dosage and in vivo mechanisms of hBN NPs remain unclear.
- This study investigates hBN NP characteristics, interactions, and toxicity.
Purpose of the Study:
- To determine the optimal dosage of hBN NPs for in vivo applications.
- To elucidate the biochemical mechanisms of hBN NP action.
- To assess acute cytotoxic effects of varying hBN NP doses.
Main Methods:
- Wistar albino rats (n=80) received intravenous hBN NPs (50-3200 µg/kg).
- Seven dosage groups and a control group were established.
- Blood and tissue samples analyzed after 24 hours.
Main Results:
- Higher hBN NP doses induced dose-dependent oxidative stress in rat serum.
- Thiol/disulfide homeostasis in kidney, liver, spleen, pancreas, and heart remained unaffected.
- Serum disulfide formation increased, disrupting thiol/disulfide balance; LOOH and MPO rose, while CAT decreased at high doses.
Conclusions:
- hBN NPs induce oxidative stress in a dose-dependent manner.
- Higher concentrations of hBN NPs modulate thiol/disulfide homeostasis.
- Findings provide crucial insights into hBN NP in vivo effects and safety.
More Related Videos
07:16Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
Published on: June 21, 2021
10:24Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry
Published on: June 7, 2018
Related Concept Videos
Preparation and Reactions of Thiols
Sulfur Assimilation
Preparation and Reactions of Sulfides
Radical Autoxidation
Oxidation of Phenols to Quinones
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox...