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Updated: Nov 11, 2025

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Molecular characterization and sequence analysis of four Brazilian rice stripe necrosis virus isolates
Daniela Domingos de Souza1, Ananias Pinto de Queiroz2, Fernando Sartori Pereira3
1Universidade Federal de Goiás, Goiânia, GO, Brazil.
Abstract:
Rice (Oryza sativa L.) is an important food crop for humanity, being cultivated in tropical and temperate regions of the world. This study reports the nearly complete genome sequences of four Brazilian rice stripe necrosis virus (RSNV) isolates. The nucleotide sequences of the RNA1 and RNA2 genome segments of these Brazilian isolates were 96.5 to 99.9% identical, indicating their close phylogenetic relationship to each other. Phylogeny and recombination analysis indicated that the genome of one of the isolates consisted of RNA segments of different origins, suggesting that a reassortment event had occurred.

