Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mismatch Repair01:36

Mismatch Repair

42.5K
Overview
42.5K
Mismatch Repair01:20

Mismatch Repair

5.7K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
5.7K
Homologous Recombination02:31

Homologous Recombination

58.5K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
58.5K
Homologous Recombination02:31

Homologous Recombination

5.4K
5.4K
Base Excision Repair01:54

Base Excision Repair

25.0K
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
25.0K
Base Excision Repair01:54

Base Excision Repair

4.5K
4.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-trajectory Bayesian modeling reveals multi-state diffusion of the MSH sliding clamp.

NPJ systems biology and applications·2026
Same author

Retroviral intasome architecture shapes the dynamics of target DNA search and integration.

PLoS pathogens·2026
Same author

An Accessible Python Framework for Real-Time Magnetic Tweezers Microscope Control and Image Processing.

bioRxiv : the preprint server for biology·2025
Same author

A high-throughput single-molecule platform to study DNA supercoiling effect on protein-DNA interactions.

Nucleic acids research·2025
Same author

A high throughput single molecule platform to study DNA supercoiling effect on protein-DNA interactions.

bioRxiv : the preprint server for biology·2024
Same author

Antagonistic roles of cGAS/STING signaling in colorectal cancer chemotherapy.

Frontiers in oncology·2024

Related Experiment Video

Updated: Nov 11, 2025

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

10.0K

Mismatch repair: Choreographing accurate strand excision.

Richard Fishel1

  • 1Department of Cancer Biology and Genetics, The Ohio State University Wexner Medical Center and James Comprehensive Cancer Center, Columbus, OH 43210, USA.

Current Biology : CB
|March 23, 2021
PubMed
Summary

State-of-the-art studies show that Saccharomyces cerevisiae Pms1 endonuclease (human PMS2) and ExoI are key players in creating strand-specific nicks essential for DNA mismatch repair.

More Related Videos

Author Spotlight: Visualizing Single-Stranded DNA During DNA Repair for Therapeutic Insights
08:30

Author Spotlight: Visualizing Single-Stranded DNA During DNA Repair for Therapeutic Insights

Published on: December 22, 2023

3.0K
Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

4.3K

Related Experiment Videos

Last Updated: Nov 11, 2025

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

10.0K
Author Spotlight: Visualizing Single-Stranded DNA During DNA Repair for Therapeutic Insights
08:30

Author Spotlight: Visualizing Single-Stranded DNA During DNA Repair for Therapeutic Insights

Published on: December 22, 2023

3.0K
Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

4.3K

Area of Science:

  • Molecular Biology
  • Genetics
  • Cellular Biology

Background:

  • DNA mismatch repair (MMR) is a crucial cellular mechanism for maintaining genomic stability.
  • Understanding the precise molecular machinery that directs MMR is essential for comprehending genome integrity.

Purpose of the Study:

  • To identify the key molecular components responsible for generating and maintaining strand-specific nicks in Saccharomyces cerevisiae.
  • To elucidate the roles of Pms1 endonuclease and ExoI in directing DNA mismatch repair.

Main Methods:

  • Utilized advanced genetic and cellular studies in Saccharomyces cerevisiae.
  • Investigated the functions of Pms1 endonuclease and ExoI in DNA repair pathways.

Main Results:

  • Demonstrated that Saccharomyces cerevisiae Pms1 endonuclease (human PMS2) and ExoI are critically involved in producing and/or maintaining strand-specific nicks.
  • These nicks precisely direct the process of mismatch repair.

Conclusions:

  • Pms1 endonuclease and ExoI are the primary effectors that establish the strand bias for DNA mismatch repair.
  • These findings provide significant insights into the fundamental mechanisms of genome surveillance and repair.