Prediction of the joint action of binary mixtures based on characteristic parameter k∙EC_x from concentration-response...

The evaluation of joint toxicity of mixtures is an important topic in toxicology. Previous studies have found that the parameter k∙EC_x of concentration response curves (CRCs) can be used to assess the applicability of concentration addition model (CA). This study further assesses the predictability of k∙EC_x on the joint toxicity evaluation. The toxicities of the twelve environmental pollutants, as well as those of binary mixtures with an equivalent-effect concentration ratio, to Vibrio fischeri were determined by using the microplate toxicity analysis. The toxicity evaluation of mixtures was conducted by CA and independent action model (IA). The relationship between the joint toxicity (measured by the relative model deviation ratio (rMDR)) and the k∙EC_x was studied. The results shows that the k∙EC_x could reflect the shape of CRCs in the whole concentration range. According to the IA and CA, 65% of the mixtures produce strong antagonistic or synergistic effect due to the significant difference of k∙EC_x. The percentage of the relative difference of k∙EC_x of components and the rMDR_x can be fitted by an exponential function. Different types of interactions could be described using this function. It is suggested that the joint toxicity of binary mixtures can be assessed with the parameter k∙EC_x, which can quickly get very important data when planning experiments, but also reduce the number of experiments.