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Updated: Nov 11, 2025
Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
Published on: June 8, 2019
Analysis of Mitochondrial DNA Control Region D-Loop in Gliomas: Result of 52 Patients
Sirin K Yuksel1, Koray Ozduman, Engin Yilmaz
1Acibadem Mehmet Ali Aydinlar University, Faculty of Arts and Sciences, Department of Molecular Biology and Genetics, Istanbul, Turkey.
Aim:
To investigate the effect of mitochondrial DNA (mtDNA) variants mainly in D-loop on glioma biology.
Material And Methods:
Sanger sequencing of D-loop (15971?16451 bp) for 52 glioma patients was performed and the variations were statistically analyzed for gender, WHO classification, morphological grade, IDH/TERT status.
Results:
Total of 122 variations (51 unique) were identified in 52 patients. C16223T, T16189C, T16311C and T16126C variants were frequently detected. The total variation number was statistically non-significant among the analyzed categories. When individual variants were considered, T16311C and T16224C were statistically significant for WHO classification (p=0.033), morphological grade (p=0.036) and gender (p=0.039), respectively.
Conclusion:
Total variation number in D-loop was not found to be related with clinical variables. Our data suggests that individual variants may play a critical role in glioma biology.
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