Hydrogen sulfide improves ox‑LDL‑induced expression levels of Lp‑PLA₂ in THP‑1 monocytes via the p38MAPK pathway

Hydrogen sulfide (H₂S) exerts an anti‑atherosclerotic effect and decreases foam cell formation. Lipoprotein‑associated phospholipase A2 (Lp‑PLA₂) is a key factor involved in foam cell formation. However, the association between H₂S and Lp‑PLA₂ expression levels with respect to foam cell formation has not yet been elucidated. The present study investigated whether H₂S can affect foam cell formation and potential signalling pathways via regulation of the expression and activity of Lp‑PLA₂. Using human monocytic THP‑1 cells as a model system, it was observed that oxidized low‑density lipoprotein (ox‑LDL) not only upregulates the expression level and activity of Lp‑PLA₂, it also downregulates the expression level and activity of Cystathionine γ lyase. Exogenous supplementation of H₂S decreased the expression and activity of Lp‑PLA₂ induced by ox‑LDL. Moreover, ox‑LDL induced the expression level and activity of Lp‑PLA₂ via activation of the p38MAPK signalling pathway. H₂S blocked the expression levels and activity of Lp‑PLA₂ induced by ox‑LDL via inhibition of the p38MAPK signalling pathway. Furthermore, H₂S inhibited Lp‑PLA₂ activity by blocking the p38MAPK signaling pathway and significantly decreased lipid accumulation in ox‑LDL‑induced macrophages, as detected by Oil Red O staining. The results of the present study indicated that H₂S inhibited ox‑LDL‑induced Lp‑PLA₂ expression levels and activity by blocking the p38MAPK signalling pathway, thereby improving foam cell formation. These findings may provide novel insights into the role of H₂S intervention in the progression of atherosclerosis.