Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tension TRAAKer: a chemigenetic fluorescent membrane tension reporter.

bioRxiv : the preprint server for biology·2026
Same author

Evidence that local viscosity and NOX-dependent ROS increases render the tardigrade H. exemplaris resilient to extreme physical force.

bioRxiv : the preprint server for biology·2026
Same author

A chemical-genetic approach to target voltage-sensitive fluorophores to mitochondria.

Mitochondrial communications·2026
Same author

Fluorogenic rhodamine B derivatives that become brighter at neutral pH.

Bioorganic & medicinal chemistry letters·2025
Same author

Membrane potential and feedback dynamics regulate CatSper-mediated progesterone signaling in human sperm.

bioRxiv : the preprint server for biology·2025
Same author

Controlling Electron Flow in Carbofluorescein Voltage Indicators.

ACS chemical biology·2025

Related Experiment Video

Updated: Nov 11, 2025

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices
12:51

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices

Published on: November 29, 2012

17.0K

Imaging Spontaneous Neuronal Activity with Voltage-Sensitive Dyes.

Benjamin K Raliski1, Molly J Kirk2, Evan W Miller1,2,3

  • 1Department of Chemistry, University of California Berkeley, Berkeley, California.

Current Protocols
|March 24, 2021
PubMed
Summary
This summary is machine-generated.

Optical imaging with VoltageFluors enables simultaneous measurement of neural circuit activity. This method offers high spatial resolution for studying neuronal excitability and connectivity in various developmental and disease models.

Keywords:
SpikeConnectVoltageFluordissociated cultureimagingspontaneous activity

More Related Videos

Functional Calcium Imaging in Developing Cortical Networks
16:33

Functional Calcium Imaging in Developing Cortical Networks

Published on: October 22, 2011

39.3K
Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye
06:43

Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye

Published on: June 20, 2019

8.0K

Related Experiment Videos

Last Updated: Nov 11, 2025

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices
12:51

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices

Published on: November 29, 2012

17.0K
Functional Calcium Imaging in Developing Cortical Networks
16:33

Functional Calcium Imaging in Developing Cortical Networks

Published on: October 22, 2011

39.3K
Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye
06:43

Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye

Published on: June 20, 2019

8.0K

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biophysics

Background:

  • Understanding neural circuit organization requires mapping cellular membrane potential changes.
  • Optical voltage sensing offers superior spatial resolution compared to electrophysiology.
  • VoltageFluors are voltage-sensitive dyes for optical neural activity studies.

Purpose of the Study:

  • To provide protocols for preparing dissociated neuronal cultures.
  • To detail methods for imaging spontaneous neuronal activity using VoltageFluors.
  • To outline data analysis techniques for optical spontaneous activity imaging.

Main Methods:

  • Preparation of dissociated rat hippocampal or cortical cultures.
  • Preparation of microisland dissociated cultures.
  • Imaging spontaneous activity in dissociated cultures using voltage-sensitive dyes.
  • Analysis of spontaneous activity imaging data.

Main Results:

  • Demonstrated the utility of VoltageFluors for high-throughput imaging of neuronal activity.
  • Established protocols for preparing and analyzing optical recordings of neuronal network function.
  • Facilitated simultaneous observation of spontaneous activity in large neuronal populations.

Conclusions:

  • Optical imaging with VoltageFluors is a powerful tool for neuroscience research.
  • These protocols support investigations into neuronal excitability and connectivity.
  • The methods are applicable to developmental studies and disease models.